Detection of HLA-specific antibodies by PRA-STAT and their association with transplant outcome

Transplantation
J E WorthingtonS Martin

Abstract

The aim was to investigate the correlation between renal transplant outcome and the presence of HLA-specific antibodies detected using the ELISA kit PRA-STAT as compared with complement-dependent cytotoxicity (CDC). 295 sera from 95 renal transplant recipients (99 transplants) were investigated for the presence of HLA-specific antibodies using both PRA-STAT and CDC. The patients were divided into group I (49 transplants failed within 1 month) and group II (50 successful transplants). The concordance between PRA-STAT and CDC for the detection of HLA class I-specific antibodies was 87.8% (259 of 295). For 19 sera, antibodies were detected only by PRA-STAT; for 17 sera, antibodies were detected only by CDC. No donor-specific antibodies were detected by either technique for patients in group II. For four group I patients (six sera), donor-specific IgG antibodies were detected only by PRA-STAT (one before, three after transplant) and all four transplants failed. For five other group I patients (six sera), donor HLA-specific antibodies were detected only by CDC (one before, four after transplant) and all five transplants failed. The antibodies detected before transplant by CDC were shown to be IgM alloantibodies. This study showed th...Continue Reading

References

Jan 1, 1993·Transplant International : Official Journal of the European Society for Organ Transplantation·A W HarmerK I Welsh
Oct 1, 1996·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·A Nanni-CostaV Bonomini

❮ Previous
Next ❯

Citations

Jul 22, 2005·Surgery Today·Junchao Cai, Paul I Terasaki
Jun 7, 2005·Human Immunology·Sylvie Di FilippoAdriana Zeevi
May 4, 2005·Human Immunology·Junchao Cai, Paul I Terasaki
Jun 2, 2000·Transplant Immunology·T HorsburghA J Robson
Oct 3, 2002·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·M Uboldi de CapeiE S Curtoni
Jun 16, 2001·Transfusion·V KiefelS Santoso
Apr 11, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Gema Fernández-FresnedoManuel Arias
Aug 8, 2008·Current Opinion in Organ Transplantation·Georg A BöhmigMarkus Wahrmann
Mar 20, 1999·Transplantation Proceedings·A C PapassavasC Stavropoulos-Giokas
May 16, 2002·Transplantation Proceedings·A C T TorrecilhasM Gerbase-DeLima
Dec 29, 1998·Current Opinion in Nephrology and Hypertension·A W Harmer
Sep 4, 2004·Clinical Transplantation·Inmaculada DíazFrancisco Valdés
Apr 1, 2006·Pediatric Transplantation·Alin L GirnitaAdriana Zeevi
Apr 3, 2007·Cytometry. Part B, Clinical Cytometry·Maria Bernadette ColomboMario Scalamogna
Jun 15, 2004·Transplantation Proceedings·E AragãoA Pacheco-Silva
Mar 8, 2000·Transplantation·R M McKennaP I Terasaki
Jul 26, 2000·Transplantation·D PlissonnierJ B Michel
May 1, 2002·Transplantation·Caner Süsal, Gerhard Opelz
Jan 31, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Bernadette MageeDerek Middleton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Paul I Terasaki
American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Paul I Terasaki, Miyuki Ozawa
© 2021 Meta ULC. All rights reserved