PMID: 7523910Oct 1, 1994Paper

Detection of human exposure to carcinogens by measurement of alkyl-DNA adducts using immunoaffinity clean-up in combination with gas chromatography-mass spectrometry and other methods of quantitation

Mutation Research
D E Shuker, H Bartsch

Abstract

A brief overview is given of recent developments from our laboratory in the use of immunoaffinity clean-up in the determination of alkyl-DNA adducts. Compound- and group-specific antibodies have been prepared against 7-alkylguanines and 3-alkyladenines. The antibodies were attached to solid supports to make immunoaffinity columns which could then be used to selectively purify either single adducts or groups of adducts prior to quantitation by various methods. In the case of methyl adducts quantitation was achieved by ELISA (3-methyl-adenine, using a monoclonal antibody) and HPLC-electrochemical detection (7-methylguanine). For groups of adducts, quantitation of the individual compounds was effected by gas chromatography-mass spectrometry (3-alkyladenines, using deuterated analogues of each adduct as an internal standard) and HPLC-fluorescence detection (7-alkylguanines). In all of these cases efficient purification of adducts from urine or DNA hydrolysates could be easily carried out. Using these techniques human exposure to alkylating agents in tobacco smoke and from cancer chemotherapy has been studied.

References

Jul 1, 1992·Chemical Research in Toxicology·D E Shuker, P B Farmer
Mar 1, 1989·Chemical Research in Toxicology·W G StillwellS R Tannenbaum
Jul 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B F ERLANGER, S M BEISER

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Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·D Tsikas
May 14, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Mihi YangFred F Kadlubar
Jul 1, 1996·Bioconjugate Chemistry·I GiraultD Molko

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