PMID: 9431922Feb 12, 1998Paper

Detection of human herpesvirus 6 DNA in serum by a microplate PCR-hybridization assay

Journal of Clinical Microbiology
C OsiowyS Zou

Abstract

PCR was performed on DNA extracts derived from clinical serum samples submitted for human herpesvirus 6 (HHV-6) serological examination. To detect amplified HHV-6 products, a hybridization-based microtiter plate assay (PCR ELISA; Boehringer Mannheim) was used. The assay system was found to be rapid, specific, and sensitive. Approximately three copies of a plasmid-based HHV-6 sequence could be detected, and no cross amplification was observed with HHV-7 genomic DNA. There was no correlation found between HHV-6 DNA detection and serological status in clinical serum samples from individuals more than 2 years old. On the other hand, in serum samples from infants less than 2 years old, a high rate of detection of HHV-6 DNA was observed in those who lacked immunoglobulin G and M antibodies to HHV-6 (55%). In this regard, PCR of serum DNA extracts may be used as a sensitive indicator of active HHV-6 infection in infants prior to their seroconversion.

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Citations

Jul 27, 2001·Pediatrics International : Official Journal of the Japan Pediatric Society·T AbeY Asano
Jan 18, 2005·Clinical Microbiology Reviews·Leen De BolleErik De Clercq
Sep 6, 2000·Clinical and Diagnostic Laboratory Immunology·S LaCroixJ B Black
Dec 23, 2000·The Journal of General Virology·S LaurentD Rasschaert
May 21, 1999·Analytical Biochemistry·B C CourtneyE A Henchal
Aug 2, 2000·Journal of Clinical Microbiology·R WahyuningsihP Schnitzler

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