Detection of human immunodeficiency virus type 1 antiretroviral resistance mutations by high-density DNA probe arrays

Journal of Clinical Microbiology
R GonzalezJ N Telles

Abstract

Genotypic resistance testing has become an important tool in the clinical management of patients infected with human immunodeficiency virus type 1 (HIV-1). Standard sequencing methodology and hybridization-based technology are the two principal methods used for HIV-1 genotyping. This report describes an evaluation of a new hybridization-based HIV-1 genotypic test of 99 clinical samples from patients infected mostly with HIV-1 subtype B and receiving treatment. This test combines RNA extraction with magnetic silica particles, amplification by nested reverse transcriptase PCR, and detection with high-density probe arrays designed to detect 204 antiretroviral resistance mutations simultaneously in Gag cleavage sites, protease, reverse transcriptase, integrase, and gp41. The nested reverse transcriptase PCR success rates at viral loads exceeding 1,000 copies/ml were 98% for the 2.1-kb amplicon that covers the Gag cleavage sites and the protease and reverse transcriptase genes, 92% for the gp41 amplicon, and 100% for the integrase amplicon. We analyzed 4,465 relevant codons with the HIV-1 DNA chip genotyping assay and the classic sequence-based method. Key resistance mutations in protease and reverse transcriptase were identified co...Continue Reading

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Citations

Jan 19, 2008·Analytical and Bioanalytical Chemistry·Michaela SchannéJörg D Hoheisel
Dec 5, 2008·Analytical and Bioanalytical Chemistry·Xuanhong ChengWilliam R Rodriguez
Feb 8, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Guy Vernet, Nathalie Tran
Aug 6, 2005·Journal of Clinical Microbiology·Jehanara KorimbocusGuy Vernet
Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Paul J JannettoSteven H Wong
Jan 3, 2016·Analytica Chimica Acta·Susana LiébanaMaría Isabel Pividori
Oct 1, 2005·Drug Discovery Today. Technologies·Thomas Jarvie
Feb 22, 2005·Journal of Microbiological Methods·Sabine CouzinetJacques Schrenzel
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Oct 21, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Guy Vernet
May 19, 2006·Expert Review of Molecular Diagnostics·Neil J Gibson
Feb 3, 2007·Expert Review of Anti-infective Therapy·Guy Vernet

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