PMID: 9524200May 9, 1998Paper

Detection of human papillomavirus in archival tissues. Comparison of in situ hybridization and polymerase chain reaction

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
E R UngerW C Reeves

Abstract

Formalin-fixed, paraffin-embedded tissues in pathology archives are an important resource for molecular epidemiology studies. Use of these tissues requires that assays be optimized to account for inevitable variations in tissue fixation and processing that occur in the performance of routine histology. We compared results of colorimetric in situ hybridization (ISH) to L1 consensus polymerase chain reaction (PCR) for detection and typing of human papillomavirus (HPV) in 180 blocks of archival tissues (up to 9 years in storage) from cervical cancer patients. Fifteen samples could not be amplified by PCR, but assays were concordant in 75.1% (124/165) of samples that could be analyzed by both methods. Similar numbers of ISH+/PCR- (23) and ISH-/PCR+ (18) cases were found. Eight of the 18 ISH-/PCR+ cases were attributable to PCR detection of HPV types not included in the ISH assay. This degree of concordance required individual optimization of assay conditions for each block. ISH and PCR assays for HPV yield complementary results, and both can be successfully applied to archival tissues.

References

Jan 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E R UngerM L Chenggis
Nov 1, 1995·The Journal of Infectious Diseases·E R UngerW C Reeves
May 1, 1995·Journal of Clinical Pathology·C S HerringtonJ O McGee
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Citations

Nov 30, 2000·Current Infectious Disease Reports·M E Hagensee
Feb 7, 2012·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Cristina PérezJulio Velasco
Aug 19, 2010·BMC Infectious Diseases·Georgette Adjorlolo-JohnsonTerence L Chorba
Dec 30, 2014·Nigerian Medical Journal : Journal of the Nigeria Medical Association·Hossein AyatollahiMaryam Sheikhi
Jan 28, 2016·Infectious Agents and Cancer·A K AwuaE K Wiredu
Nov 4, 2000·Respiratory Medicine·J Bousquet
Jul 25, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Enrique SantiagoFernando Vázquez
Nov 22, 2005·Journal of Medical Virology·Janine T BryanDarron R Brown
Jul 17, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·P BirnerG Oberhuber
Mar 29, 2005·Experimental Biology and Medicine·A TsezouN Vamvakopoulos

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