PMID: 8599431Jun 1, 1995Paper

Detection of human papillomavirus in extragenital Bowen's disease using in situ hybridization and polymerase chain reaction

The American Journal of Dermatopathology
G CollinaG P Trentini

Abstract

Extragenital Bowen's disease (EBD) has rarely been studied for the presence of human papillomaviruses (HPVs). Twenty consecutive patients with EBD were investigated for the presence of HPVs using in situ hybridization with a generic probe that can detect HPV DNA types 6, 11, 16, 18, 30, 31, 33, 35, 45, 51, and 52 and specific probes for HPV DNA types 6/11, 16/18, and 31/33/35. All cases were tested with the polymerase chain reaction (PCR) technique employing the L1 consensus primer pair, MY11 (primer for the positive strand) and MY9 (primer for the negative strand) complementary to genital and dermal HPV types. Seven caucasian patients, five males and two females, with an average age of 70.4 years, showed positive in situ hybridization (ISH) for HPV DNA. The positivity varied from 5 to 40% of neoplastic cells. Three of seven of the ISH DNA-positive cases showed a positive PCR for DNA HPVs. The role of HPVs in human tumors is not fully understood since oncogenic types of HPVs have been found in normal tissue and the actions of cofactors have been postulated. Bowen's disease usually occurs in elderly people in whom the efficiency of the immune systems may be compromised. The association between HPV infection and low efficiency of...Continue Reading

Citations

Jul 1, 1997·The Journal of Pathology·A M McNicol, M A Farquharson
Jun 10, 2000·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·G Petter, U F Haustein
Sep 28, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D J WileyLynne Fukumoto
Oct 3, 2006·The American Journal of Dermatopathology·Joannie D Sun, Ronald J Barr
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Dec 3, 1999·The British Journal of Dermatology·N H CoxC A Morton
Feb 26, 2016·The American Journal of Dermatopathology·Marián ŠvajdlerMichal Michal
Mar 17, 2001·The British Journal of Dermatology·T MitsuishiT Sata
Aug 15, 2015·Journal of Cutaneous Pathology·Munir H IdrissAlmut Böer-Auer

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