Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology.

Journal of Clinical Microbiology
C BiswasJ M Best

Abstract

Human papillomavirus type 16 (HPV-16) is associated with abnormal Papanicolou smears, indicative of cervical intraepithelial neoplasia. HPV-16 is the most common genital HPV and is found in up to 40% of young women with normal cervical cytology. In order to investigate whether transcriptionally active HPV-16 infection is associated with abnormal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading frame was developed to detect all HPV-16 early-region mRNA (E-mRNA) transcripts. It was used to study HPV-16-infected women with normal and abnormal cervical cytologies to obtain evidence of active infection. Among HPV-16 DNA-positive women, HPV-16 E-mRNA was detected in 15 of 37 (40.5%) women with abnormal cervical cytology but in only 4 of 35 (11.4%) women with normal cytology (P = 0.007). Thus, HPV-16 E-mRNA transcription is associated with abnormal cervical smears and may have value as a prognostic marker of progressive disease.

References

Feb 1, 1992·Human Pathology·M H StolerT R Broker
Jul 11, 1992·Lancet·J CuzickM Anderson
Jul 11, 1991·Nucleic Acids Research·R T D'AquilaJ C Kaplan
Jan 25, 1990·Nucleic Acids Research·C Gaillard, F Strauss
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D J McCanceL A Laimins
Mar 1, 1995·Baillière's Clinical Obstetrics and Gynaecology·M I Shafi, D M Luesley
May 1, 1995·Journal of Clinical Microbiology·T J BosmaJ M Best
Sep 30, 1993·International Journal of Cancer. Journal International Du Cancer·E M HsuM Paraskevas
Aug 1, 1994·Biochemical Society Transactions·B KellJ M Best
Jan 1, 1994·Intervirology·P G Fuchs, H Pfister
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Jeon, P F Lambert

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Citations

Dec 14, 2011·Archives of Gynecology and Obstetrics·Krishnakumar VinodhiniKalimuthusamy Natarajaseenivasan
Oct 14, 2003·Journal of Medical Virology·Christine MantJohn Cason
Nov 18, 2006·Virchows Archiv : an International Journal of Pathology·Ujjal Kumar BhawalHiroki Kuniyasu
Jun 18, 1999·Reviews in Medical Virology·P S RiceJ E Banatvala
Apr 4, 2000·Journal of Medical Virology·P S RiceJ M Best
Mar 9, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·John Cason, Christine A Mant

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