Detection of human parvovirus B19 in papillary thyroid carcinoma.

British Journal of Cancer
J H WangG S Huang

Abstract

To evaluate whether parvovirus B19, a common human pathogen, was also involved in papillary thyroid carcinoma (PTC), 112 paraffin-embedded thyroid specimens of benign nodules, papillary, medullary and follicular carcinomas, and normal controls were examined for B19 DNA and capsid protein by nested PCR, in situ hybridisation (ISH) and immunohistochemistry (IHC). The expression of the nuclear factor-kappaB (NF-kappaB) was investigated by IHC. The results showed B19 DNA commonly exists in human thyroid tissues; however, there were significant differences between PTC group and normal controls, and between PTC and nonneoplastic adjacent tissues (P<0.001). The presence of viral DNA in PTC neoplastic epithelium was confirmed by laser-capture microdissection and sequencing of nested PCR products. B19 capsid protein in PTC group was significantly higher than that of all the control groups and nonneoplastic adjacent tissues (P<or=0.001). Compared with control groups, the activation of NF-kappaB in PTC group was significantly increased (P<or=0.02), except for medullary carcinomas, and the activation of NF-kappaB was correlated with the viral protein presence (P=0.002). Moreover, NF-kappaB was colocalised with B19 DNA in the neoplastic epi...Continue Reading

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Citations

Dec 31, 2010·Thyroid : Official Journal of the American Thyroid Association·Laura A AdamsonJacqueline A Hobbs
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ashkan EtemadiMohsen Moghoofei
May 11, 2010·The Journal of Pathology·Kirk JensenVasyl Vasko
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Mar 29, 2014·Endocrine Pathology·Laura A Adamson-SmallJacqueline A Hobbs
Jan 11, 2020·Molecular Medicine Reports·Chia-Cheng SuBor-Show Tzang
Nov 23, 2013·Journal of Medical Virology·Laura A AdamsonJacqueline A Hobbs
Dec 11, 2019·Medicina·Sabine GravelsinaModra Murovska
May 26, 2016·Reviews in Endocrine & Metabolic Disorders·Poupak FallahiAlessandro Antonelli
Apr 5, 2017·Frontiers in Endocrinology·Silvia Martina FerrariSalvatore Benvenga
Sep 30, 2014·The International Journal of Biological Markers·Dimitris StamatiouDemetrios A Spandidos
Nov 22, 2018·Frontiers in Microbiology·Merle HöpkenVerena Schildgen
Jun 21, 2018·Frontiers in Cellular and Infection Microbiology·Safder S Ganaie, Jianming Qiu

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Methods Mentioned

BETA
PCR
laser-capture microdissection
nuclear translocation
laser-capture

Software Mentioned

SPSS
BLAST
Statistical Program for Social Sciences ( SPSS )

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