Detection of human T-lymphotropic virus type-I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT-PCR/ISH)

International Journal of Cancer. Journal International Du Cancer
K OhshimaM Kikuchi

Abstract

To examine the relationship between human T-lymphotrophic virus type I (HTLV-I) proviral DNA and its expression in the lymph nodes, HTLV-I DNA and tax/rex mRDA were directly amplified by polymerase chain reaction in situ hybridization (PCR/ISH), and reverse transcription (RT)-PCR/ISH [RT-PCR/ISH]. We studied 24 lymph nodes from patients with adult T-cell leukemia/lymphoma (ATLL), incipient ATLL (I-ATLL), and HTLV-I associated lymphadenitis dermatopathic type (HAL-D) and enlarged paracortical type (HAL-EP). In ATLL, 40-60% of the nucleated cells were positive for for HTLV-I proviral DNA by PCR/ISH, while in I-ATLL and HAL, respectively 5-20% and less than 1-5% of cells were positive. The number of mRNA expressing cells was smaller than that of the proviral DNA-positive cells. The mRNA-expressing cells varied in number among the ATLL and I-ATLL cases, while they were only rarely observed in HAL-D and HAL-EP. These results show that HTLV-I infection and activation might increase with malignant transformation of the target T helper cells.

References

Jun 9, 1988·Nature·R GalloM Yoshida
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M FujiiI M Verma
Jun 9, 1988·Nature·J D Kalbfleisch, J F Lawless

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Citations

Mar 3, 2004·International Journal of Cancer. Journal International Du Cancer·Satoshi TakedaMasao Matsuoka
Apr 29, 2006·International Journal of Cancer. Journal International Du Cancer·Misuzu ShimakageMasuo Yutsudo
Aug 7, 1997·International Journal of Cancer. Journal International Du Cancer·K OhshimaM Kikuchi
Jun 8, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·N TakenouchiS Izumo

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