Detection of hyperdiploid karyotypes (>50 chromosomes) in childhood acute lymphoblastic leukemia (ALL) using fluorescence in situ hybridization (FISH)

Leukemia
J RitterbachF Lampert

Abstract

ALL patients with a hyperdiploid karyotype of more than 50 chromosomes (high hyperdiploidy) carry a better prognosis in contrast to patients presenting with other cytogenetic features, and an appropriate less intensive therapy protocol should be developed for these patients. For this reason it is desirable to have a quick screening method identifying those with this type of hyperdiploidy. We therefore studied the bone marrow and/or blood cells of 278 children with ALL using double target fluorescence in situ hybridization (FISH) on interphase. A combination of DNA probes (repetitive, centromere specific) was applied detecting chromosomes which are most frequently overrepresented in patients with hyperdiploidy (>50), at chromosomes 6, 10, 17 and 18. All patients showing hybridization signals differing from the normal signal distribution of two spots for each tested chromosome were analyzed cytogenetically as well. 102 children (102/278; 36.7%) were found to have a clone with aberrant FISH results. In 80 patients (80/278, 28.8%) the cytogenetic analysis detected a hyperdiploid karyotype >50 chromosomes, whereas the remaining patients (n=12) could be related to other ploidy subgroups, ie hyperdiploidy with 47-50 chromosomes, haplo...Continue Reading

Citations

Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka
Jan 13, 2001·Cancer Genetics and Cytogenetics·J A van den BergheS H Lee
Apr 28, 2004·Journal of Pediatric Hematology/oncology·Patricia Pérez-VeraRocío Ortíz
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May 6, 2009·Genes, Chromosomes & Cancer·Kajsa Paulsson, Bertil Johansson
Oct 2, 2009·International Journal of Laboratory Hematology·P Rachieru-SourisseauA Chassevent
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