PMID: 6406602May 27, 1983Paper

Detection of IgD 'hidden' lambda light chain

Journal of Immunological Methods
G SpiraA Carter

Abstract

Immunoelectrophoresis and immunodiffusion of some IgD myeloma proteins fail to demonstrate precipitin bands between the light chain and anti-light chain serum. Reduction and alkylation of purified IgD preparation was found to render the molecule reactive to anti-lambda serum. Iodinated IgD myeloma protein was successfully precipitated by both anti-delta and anti-lambda antibodies. Both antisera precipitated heavy and light chains of a MW of 70,000 and 24,000 daltons respectively. In addition, a band of 39,000 daltons was also evident. Reduction and alkylation of the iodinated molecule, followed by precipitation with anti-delta serum demonstrated only the 70,000 and 39,000 daltons bands. The present report indicates that by sensitive techniques, such as iodination and immunoprecipitation, non-reactive hidden light chains can be detected in IgD lambda molecules.

References

Jan 1, 1977·Immunological Reviews·R Jefferis, J B Matthews
Jan 1, 1975·Archives of Internal Medicine·M Seligmann
Dec 31, 1971·Annals of the New York Academy of Sciences·M SeligmannB Frangione
Jan 1, 1965·The Journal of Experimental Medicine·D S ROWE, J L FAHEY
Jan 1, 1965·The Journal of Experimental Medicine·D S ROWE, J L FAHEY

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