PMID: 1198069Jan 1, 1975Paper

Detection of IgG aggregates or immune complexes using solid-phase C1q and protein A-rich Staphylococcus aureus as an indicator system

Scandinavian Journal of Immunology
C FarrellS E Svehag

Abstract

A radioimmunoassay for detection of C1q-binding IgG aggregates and antigen-IgG antibody complexes is described. The assay makes use of solid-phase C1q and 32p-labelled protein A-rich Staphylococcus aureus as an indicator system. Both 19S and heavier IgG aggregates that fixed C1q were detected. The sensitivity of the assay permitted detection of heavy (19-25S) IgG aggregates at a concentration of 8 mug/ml or less. The results indicated that detection of IgG in this assay is dependent on the degree of IgG polymerization and the molar ratio between the solid-phase C1q and the IgG polymers. Albumin-anti-albumin complexes, preformed at equilibrium with antibody to antigen molar ratios of 2:1 to 3:1 and at antigen concentrations of 25 to 40 mug/ml, were also detectable using the described radioimmunoassay.

References

Nov 1, 1973·Postgraduate Medicine·J H Vaughan
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Jan 1, 1974·Scandinavian Journal of Immunology·C EndresenA Grov
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Nov 1, 1971·Immunochemistry·W AugenerH J Müller-Eberhard
Feb 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·V I OYAMA, H EAGLE

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Citations

Jun 1, 1978·Arthritis and Rheumatism·R C Williams, A D Bankhurst
Jan 1, 1979·Preparative Biochemistry·C GranierM Steinbuch
Jul 1, 1978·Acta paediatrica Scandinavica·H E NielsenN Høiby
Apr 1, 1981·Journal of Clinical Pathology·S M Walker, S F Al Khateeb
Apr 1, 1979·The Journal of Clinical Investigation·J S McDougalC L Christian
Jan 1, 1979·Scandinavian Journal of Immunology·A M Teppo, O Wager
Sep 15, 1976·International Journal of Cancer. Journal International Du Cancer·R Heimer, G Klein
Jan 1, 1984·Cancer Immunology, Immunotherapy : CII·P M Lutz, J R Dawson
Aug 1, 1976·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·H Nielsen, S R Svehag
Mar 29, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D P Dos SantosR A A da Silva
Jan 1, 1980·Gastroenterologia Japonica·T ArimaH Nagashima
Feb 1, 1976·Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology·S E Svehag, D Burger

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