PMID: 2109199Jan 1, 1990Paper

Detection of IgG and IgE antibodies to Aspergillus fumigatus in human sera by immunogold assay

Mycopathologia
H C GugnaniJ N Fink

Abstract

An immunogold assay (IGA) was developed to detect IgG and IgE antibodies to Aspergillus fumigatus. Sixteen sera from patients with allergic bronchopulmonary aspergillosis (ABPA), aspergilloma, and normal controls were studied. All sera were also evaluated for antibodies against A. fumigatus by biotin-avidin linked enzyme immunosorbent assay (BALISA) and by agar gel double diffusion method. A. fumigatus specific IgG and IgE antibodies could be detected by IGA in all the patients' sera but not in the sera of normal controls. Both IgG and IgE antibodies to A. fumigatus could be demonstrated in all the sera by BALISA and normal controls showed only low levels of these antibodies. There was a positive correlation between the degree of reactivity detected by IGA, the BALISA titer and the precipitins by agar gel diffusion. It can be concluded that IGA is a reliable, sensitive and simple method capable of detecting both IgG and IgE antibodies against A. fumigatus in patient serum.

References

Aug 1, 1977·Journal of Clinical Microbiology·M A GordonJ Kane
Oct 1, 1977·The Journal of Allergy and Clinical Immunology·J N FinkG H Scribner
Jan 1, 1986·International Archives of Allergy and Applied Immunology·V P KurupJ N Fink
Jul 1, 1986·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·V P Kurup
Dec 1, 1986·The Journal of Allergy and Clinical Immunology·V P KurupJ N Fink
Jan 1, 1988·International Archives of Allergy and Applied Immunology·V P KurupJ N Fink

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Citations

Jan 8, 2010·International Journal of Molecular Sciences·David A EdmondsonJordan N Fink
May 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K E ReijulaJ N Fink
May 17, 2015·Medical Mycology·Iain D PageDavid W Denning
Oct 1, 1991·Clinical Microbiology Reviews·V P Kurup, A Kumar

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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