Detection of IgG binding to Schistosoma mansoni recombinant protein RP26 is a sensitive and specific method for acute schistosomiasis diagnosis

Parasitology International
E MakarovaA M Goes

Abstract

We recently described the first recombinant Schistosoma mansoni protein RP26, which was capable of acute infection diagnosis. The aim of the present work was to further characterize the RP26 diagnostic properties in immunoblot and enzyme-linked immunosorbent (ELISA) assays. Testing sera from uninfected donors and sera from patients with acute or chronic Schistosoma infection by Western blot immunoassay revealed 100% specificity and 100% sensitivity for acute infection identification. Sera from uninfected, acute, and chronic schistosomiasis were also probed for IgG, IgG4, IgA, and IgM reactivity to RP26 plus soluble egg antigens (SEA) in ELISA. The mean IgG reactivity to RP26 by sera from acute schistosomiasis patients was significantly higher than the chronic ones. The IgG4, IgA, and IgM reactivities to RP26 were low and similar in both infected groups. The mean IgA and IgM reactivities to SEA were significantly higher in the group of acute compared to chronic group, whereas mean IgG4 reactivity was higher in chronic group. To estimate the specificity of Schistosoma infection diagnosis sera from patients infected with other different parasites were tested to detect IgG reactivity to RP26 and IgA and IgM reactivity to SEA. For I...Continue Reading

Citations

Oct 15, 2008·Acta Tropica·Lílian BeckSilvia M L Montenegro
Aug 21, 2015·PLoS Neglected Tropical Diseases·Chihiro TanigawaSatoshi Kaneko
May 24, 2018·Medical Sciences : Open Access Journal·Olumide AjibolaEmmanuel Obishakin
Aug 1, 2015·Clinical Microbiology Reviews·Kosala G A D WeerakoonDonald P McManus
Apr 20, 2021·Acta Tropica·Silvia Stefania LongoniZeno Bisoffi

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