PMID: 6403624Mar 11, 1983Paper

Detection of immune complexes by their complement-dependent binding to bovine conglutinin: technical aspects of a solid-phase immunoenzyme microassay and its application to normal and pathological sera

Journal of Immunological Methods
T RaffinJ P Lamelin

Abstract

A micromethod for the solid-phase conglutinin binding assay (Con BA) for the detection of circulating immune complexes (CIC) is described, in which the use of microplates, glucose oxidase-coupled anti-human immunoglobulins and automatic OD recorder contributed to the speed and low cost of this reproducible and sensitive test. The Con BA values of a large population of healthy blood donors had a wide distribution which in our series of 189 appeared to be trimodal. The Con BA values clearly reflected the levels of the main Ig classes (M, G, and A). This was confirmed by follow up of 6 individuals with high initial Con BA values. For 4 of them, a parallel decrease in Con BA value and IgG concentration was observed. In the 2 others, the sustained high level of Con BA remained unexplained. Complement components and activity of these sera were within normal limits. Because of fluctuation in the aggregated human gamma-globulins (AHG) reference curve, expression of results was based upon the standard deviation of 6 normal sera. In view of the above results, these sera were selected from those with the lowest Con BA values. On the basis that Con BA and C1q BA may detect CIC differing in their ag/ab ratio, sera from rheumatoid arthritis ...Continue Reading

References

Jan 1, 1976·The Journal of Clinical Investigation·A N TheofilopoulosF J Dixon
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A R Neurath, N Strick
Feb 1, 1977·Annals of the Rheumatic Diseases·H R Schumacher
Nov 6, 1974·Biochemical and Biophysical Research Communications·S C Lee, L Levine
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
May 15, 1981·International Journal of Cancer. Journal International Du Cancer·R B HerbermanA Theofilopoulos

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Citations

Jun 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·E Rødahl

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