PMID: 6167639Jan 1, 1981Paper

Detection of immune deposits in glomeruli: the masking effect on antigenicity of formalin in the presence of proteins

Journal of Immunological Methods
J Hed, S Eneström

Abstract

Formalin is known to mask the antigenicity of immune deposits in glomeruli but not of surface immunoglobulins of isolated lymphocytes. We have shown in mice with experimental passive anti-GBM glomerulonephritis that formalin masks the antigenicity of GBM-bound immunoglobulins only if the tissue is fixed before sectioning. The presence of a high concentration of normal bovine serum during fixation of cryostat sections masks the antigenicity of immune deposits, whereas formalin alone has no obvious effect. The same results were obtained with human immunoglobulins (IgG, IgM and IgA) bound to tissue sections. Protease treatment with pepsin and trypsin restored the ability of the immunoglobulins to be stained. The masking effect seems to be due to extensive cross-linking of environmental proteins which prevents fluorescent conjugates reaching their antigens. Methods for detecting immunoglobulins in tissues must, therefore, take into consideration the influence of fixatives not only on epitopes but also on the environment in which the antigenic determinants are localised.

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Citations

Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·R RüchelU Helmchen
Feb 25, 1983·Journal of Immunological Methods·J Hed, S Eneström
Nov 13, 1989·Journal of Immunological Methods·G HalldénS G Johansson
Jan 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·O EvensenA Nilsen
Nov 1, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Carla Rossana ScaliaGiorgio Cattoretti
Nov 1, 1983·The Journal of Pathology·E L Jones

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