Detection of increased tissue concentrations of nerve growth factor with an improved extraction procedure

Journal of Neuroscience Research
C ZettlerR A Rush

Abstract

Nerve growth factor (NGF) is a protein essential for the survival and normal function of sympathetic neurons. Two-site immunoassays have been developed over the past decade in several laboratories and used to estimate its endogenous concentrations in a variety of effector tissues. However, levels appear restricted to a narrow range, display only a poor correlation with innervation density, and show obvious inter- and intralaboratory variations, the origins of which are unclear. This led us to examine alternative extraction procedures for NGF before quantification. In particular, we have found treatment of tissue extracts with high and low pH in the presence of detergent results in the detection of higher NGF concentrations in immunoassays using either polyclonal or commercially available monoclonal antibodies. These increases were tissue-specific (sciatic nerve, mesenteric arteries, and thoracic aorta > heart and brain > sympathetic ganglia > abdominal aorta) and as much as 10 times greater than the amounts detected by traditional procedures. The method should also prove useful for the assay of other members of the neurotrophin family when appropriate antibodies become available.

References

Aug 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·D J MacDonald, A M Kelly
Mar 15, 1979·Biochemical and Biophysical Research Communications·H RonneP A Peterson
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K SudaH Thoenen
Mar 1, 1992·Journal of Hypertension·T UeyamaS Furukawa
Mar 1, 1992·Arthritis and Rheumatism·L AloeR Levi-Montalcini
Jan 11, 1991·Brain Research·C ZettlerR A Rush
Mar 1, 1989·Journal of Neuroscience Research·P H Koo, R W Stach
Jan 1, 1987·Journal of Neuroscience Research·R W Stach, J R Perez-Polo
Oct 1, 1987·Journal of Neurochemistry·R W StachJ R Perez-Polo
Feb 26, 1987·Journal of Immunological Methods·L Lärkfors, T Ebendal
Jan 1, 1987·Methods in Enzymology·S Korsching, H Thoenen
Sep 4, 1987·Science·R Levi-Montalcini
Oct 1, 1980·Physiological Reviews·H Thoenen, Y A Barde
Aug 1, 1981·Journal of Neurochemistry·E J OlenderR W Stach
Mar 1, 1983·Journal of Cellular Physiology·C E Chandler, H R Herschman
Sep 15, 1983·European Journal of Biochemistry·A CattaneoP Calissano
Mar 1, 1983·Journal of Neurochemistry·S FurukawaK Hayashi
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Korsching, H Thoenen
Sep 1, 1984·The Journal of Cell Biology·E M BarthH Thoenen
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D L Shelton, L F Reichardt
May 1, 1983·The Journal of Physiology·C E HillD F van Helden
Mar 28, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·K KatoA Kosaka
Mar 1, 1994·Journal of Neuroscience Research·J NanduriK E Neet

❮ Previous
Next ❯

Citations

Jan 1, 2014·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kamini DangatSadhana Joshi
Aug 20, 2008·BMC Research Notes·Kakarla V ChalamRavi K Murthy
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Jihong XingJianhua Li
Jun 7, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Jiahao LiuJianhua Li
Feb 3, 2007·Neurourology and Urodynamics·Lori A BirderNeil M Resnick
Mar 14, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Akira FurutaNaoki Yoshimura
Aug 1, 1997·Clinical and Experimental Pharmacology & Physiology·R A RushX F Zhou
May 16, 2003·Journal of Biomaterials Science. Polymer Edition·Terri Adams Kapur, Molly S Shoichet
Sep 25, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Anitha KilariSadhana Joshi
Mar 23, 2012·Journal of Neuropathology and Experimental Neurology·Kristine L P GarciaMargaret Fahnestock
Sep 26, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Angela M BetancourtRussell L Carr
Jan 17, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Madhavi DhobaleSadhana Joshi
Sep 8, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Vandita D'souzaSadhana Joshi
Oct 2, 1998·The American Journal of Physiology·D B ClemowJ B Tuttle
Jan 23, 2013·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kamini DangatSadhana Joshi
Jun 1, 1997·Experimental Neurology·A J Okragly, M Haak-Frendscho
Feb 23, 2000·Journal of the Autonomic Nervous System·M M BrauerK A Crutcher
Sep 7, 1999·Journal of Neuroscience Methods·S H ZhangR A Rush
Jun 17, 1998·Brain Research·J M SpitsbergenJ B Tuttle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.