PMID: 2506638Sep 1, 1989Paper

Detection of individual interleukin 4- and gamma interferon-producing murine spleen cells after activation with T-cell mitogens

Scandinavian Journal of Immunology
B SanderG Möller

Abstract

Murine spleen cells were activated with concanavalin A (Con A), pokeweed mitogen (PWM), or phorbol myristate acetate (PMA) and the calcium ionophore A23187. Cells producing gamma interferon (IFN-gamma) or interleukin 4 (IL-4) could be detected by lymphokine-specific monoclonal antibodies and indirect immunofluorescence. The frequency and kinetics of the lymphokine-producing cells were examined and were approximately the same after stimulation with Con A or PMA and A23187. Thirty hours after activation, 3-9% of the cells produced IFN-gamma. There were few IL-4-producing cells, and the maximal frequency was 1 out of 400 spleen cells 48 h after activation. When the cells were activated with PWM, the frequency of IFN-gamma-producing cells was still high 72 h after culture. The majority of the IFN-gamma-producing cells were CD8+ and expressed receptors for IL-2.

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Citations

Jan 1, 1993·Viral Immunology·J RubyI Ramshaw
Jan 1, 1994·Scandinavian Journal of Immunology·M D Leite de MoraesM Hontebeyrie-Joskowicz
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·A V Gett, P D Hodgkin
Jul 12, 2005·Journal of Neuroimmunology·Emma H WilsonChristopher A Hunter
Aug 1, 1991·European Journal of Immunology·B SanderE Möller

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