Detection of infectious laryngotracheitis virus in formalin-fixed, paraffin-embedded tissues by nested polymerase chain reaction

Avian Diseases
Jennifer HumberdThomas P Brown

Abstract

Infectious laryngotracheitis virus (ILTV) is routinely diagnosed by histopathologic examination of trachea, eyelid, and lung tissues. Lesions consistent with infectious laryngotracheitis (ILT) infection include syncytial cell formation with intranuclear inclusion bodies. These changes are present during the acute phase of infection. To increase the sensitivity of detecting ILT, a nested polymerase chain reaction (PCR) was developed for detection of ILTV DNA. Nested PCR assay was specific for the amplification of ILTV DNA and did not amplify a variety of other avian pathogens. To further validate the ability of this assay to detect ILT, nested PCR was performed in formalin-fixed, paraffin-embedded tissues from 35 cases of respiratory disease. Of the 35 cases, 12 were considered ILT suspects on the basis of initial clinical observation. Eleven of the 12 ILT-suspect cases were diagnosed as ILT, and the remaining 24 were diagnosed as nonspecific tracheitis (NST) by histopathologic examination. Histopathologically positive samples were confirmed by direct fluorescent antibody test and virus isolation. Of the 11 ILT-positive cases, 10 were positive by nested PCR. In addition, ILTV DNA was detected in 7 of the 24 samples diagnosed as ...Continue Reading

References

Jan 1, 1991·Archives of Virology·C S HughesR C Jones
Jun 1, 1972·Australian Veterinary Journal·A J Turner
Sep 1, 1995·Avian Pathology : Journal of the W.V.P.A·T J Bagust, M A Johnson
Jan 1, 1988·Avian Pathology : Journal of the W.V.P.A·J J York, K J Fahey

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Citations

Jul 31, 2004·Avian Diseases·Holly S SellersJames S Guy
Feb 7, 2008·Avian Diseases·Andrés Rodríguez-AvilaMaricarmen García
Oct 23, 2008·Avian Diseases·A A OwoadeC P Muller
Jul 1, 2008·Journal of Virological Methods·Jorge Luis Chacón, Antonio J Piantino Ferreira
Jan 28, 2014·Avian Pathology : Journal of the W.V.P.A·Kimberly R MenendezNathaniel L Tablante
Nov 1, 2013·World Journal of Virology·Shan-Chia Ou, Joseph J Giambrone
May 26, 2018·British Poultry Science·Silvana Santander ParraAntonio J Piantino Ferreira
Dec 29, 2020·The Analyst·Mohamed El-TholothHaim H Bau

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Methods Mentioned

BETA
scraping
enzyme-linked immunosorbent assay
PCR
nucleic acid extraction
scrapings
light microscopy

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