Detection of influenza A and B with the Alere ™ i Influenza A & B: a novel isothermal nucleic acid amplification assay

Influenza and Other Respiratory Viruses
Briony HazeltonJen Kok

Abstract

Rapid influenza diagnostic tests (RIDTs) have an important role in clinical decision-making; however, the performances of currently available assays vary widely. We evaluated the performance of the Alere(™) i Influenza A&B (Alere(™) iNAT), a rapid isothermal nucleic acid amplification assay that has recently received FDA clearance, for the detection of influenza A and B viruses during the Australian influenza season of 2013. Results were compared to two other RIDTs tested in parallel; Quidel Sofia(®) Influenza A+B fluorescent immunoassay (FIA) and Alere(™) BinaxNOW(®) Influenza A & B immunochromatographic (ICT) assay. A total of 202 paired nasopharyngeal swabs collected from patients ≥ 16 years old with an influenza-like illness (ILI) were eluted in 2 ml of universal transport medium (UTM) that was used to perform all three RIDTs in parallel. Reverse-transcription polymerase chain reaction (RT-PCR) was used as the reference standard. Compared to RT-PCR, Alere(™) iNAT detected 77.8% influenza A positive samples versus 71.4% and 44.4% for the Quidel Sofia(®) Influenza A+B FIA and BinaxNOW(®) Influenza A & B ICT assay, respectively. For influenza B, Alere(™) iNAT detected 75% of those positive by RT-PCR, versus 33.3% and 25.0% for...Continue Reading

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Methods Mentioned

BETA
nucleic acid amplification
electrophoresis

Software Mentioned

Sofia
Alere iNAT
Alere

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