PMID: 9187695Jun 1, 1997Paper

Detection of intact prostate cancer cells in the blood of men with prostate cancer

Urology
Paul O P Ts'oA W Partin

Abstract

To develop a procedure to be used to find, identify, and characterize the living prostate cancer cells in the blood of patients with prostate cancer. The procedure is based on a negative selection approach that removes most of the blood cells and collects the remaining prostate cancer cells, which are identified and characterized by fluorescent in situ hybridization with deoxyribonucleic acid probes and by indirect fluorescent immunocytochemical staining. The blood cells are removed via density gradient centrifugation. Using the prostate cancer LNCaP cells as a model, the recovery rate of the added prostate cancer cells to 10 mL of blood was about 85%, with a dilution of 1 LNCaP cell to 10,000 white blood cells or more. Blood samples varying from 9 to 27 mL were collected and analyzed from 8 men aged 54 to 79 years who had varying levels of PSA in serum. In one blood sample, prostate cancer cells were not found; in the seven other samples, the number of prostate cancer cells found per milliliter of blood varied from 1 to 20. Prostate cancer cells were not found in 7.5 to 15-mL blood samples from 3 healthy younger men. The prostate cells were found to be aneuploid for chromosomes 7 and 8, highly suggestive that these cells were ...Continue Reading

References

Nov 1, 1994·The Journal of Urology·A W Partin, J E Oesterling
Dec 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M V SeidenW DeWolf
May 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R A GhosseinJ Rosai
Jun 28, 1995·Journal of Immunological Methods·C GriwatzK S Zänker
Apr 28, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Vincenzo EspositoAntonio Chirianni

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Citations

Sep 24, 1999·Journal of Clinical Laboratory Analysis·A BenezG Fierlbeck
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·V Uma BaiG Prem-Veer Reddy
Dec 8, 2007·Breast Cancer Research and Treatment·Katherine H Rak TkaczukPaul O P Ts'o
Apr 19, 2005·Urology·Jose G MorenoLeon W M M Terstappen
Jul 17, 2004·Neoplasia : an International Journal for Oncology Research·Robert D LobergKenneth J Pienta
Feb 3, 2004·Journal of Cellular Biochemistry·Masashi NakayamaWilliam G Nelson
Mar 3, 2004·Journal of Cellular Biochemistry·Magnus EdlundLeland W K Chung
Apr 13, 2000·Cancer Control : Journal of the Moffitt Cancer Center·J G Moreno, L G Gomella

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