Detection of integrons and antibiotic-resistance genes in Salmonella enterica serovar Typhimurium isolates with resistance to ampicillin and variable susceptibility to amoxicillin-clavulanate

International Journal of Antimicrobial Agents
María Luisa GüerriRafael Rotger

Abstract

We characterized 29 antimicrobial-resistant Salmonella enterica serovar Typhimurium strains, including four belonging to the monophasic variant 4,5,12:i:-, mostly isolated from infants. They were selected from 3230 strains isolated in the years 1990-2001 on the basis of resistance to ampicillin and variable susceptibility to the amoxicillin-clavulanate combination. Twenty-three strains were resistant to more than four antibiotics. All the strains carried the bla(TEM) gene and most were able to transfer this gene by conjugation. Sequencing of the gene from one of the amoxicillin-clavulanate-resistant strains allowed identification of the encoded beta-lactamase as TEM-1; all of these strains carried a second gene encoding beta-lactamase production, either pse-1 or oxa1. However, the association of bla(TEM) plus pse-1 genes did not always confer resistance to amoxicillin-clavulanate. The pse-1 gene, found in 17 strains, was located in the Salmonella Genomic Island-1 (SGI1), which carries two integrons and encodes multiple drug-resistance. None of the oxa1-bearing strains had the SGI1, yet this gene was found as part of an integron that also carried the aadA1 gene and was not plasmid-associated. Thirteen of the strains harbouring S...Continue Reading

Citations

Jul 14, 2010·The Journal of Veterinary Medical Science·Hesham DahshanKaroku Okamoto
Apr 27, 2013·Enfermedades infecciosas y microbiología clínica·María de ToroYolanda Sáenz
Nov 16, 2006·Journal of Applied Microbiology·J Bauer-GarlandP J Fedorka-Cray
Nov 22, 2012·Journal of Applied Microbiology·J L ThomasW D Taylor
Aug 19, 2015·Enfermedades infecciosas y microbiología clínica·Ana González-TorralbaJuan-Ignacio Alós
Jan 8, 2015·Environmental Science and Pollution Research International·Chao-Yu HsuYu-Li Huang
Jul 30, 2014·The Science of the Total Environment·Chi-Wei TaoYu-Li Huang
Feb 17, 2009·Enfermedades infecciosas y microbiología clínica·María Del Carmen MendozaMaría Rosario Rodicio
Feb 14, 2018·International Journal of Environmental Research and Public Health·Terence OdochYngvild Wasteson
Oct 9, 2008·The Journal of Veterinary Medical Science·Pawin PadungtodGlen Hill

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