Jun 1, 1989

Detection of interleukin 1 with human dermal fibroblasts

H LoppnowE Brandt


A fibroblast proliferation assay was developed for the detection of interleukin 1 (IL 1). Proliferation was measured by thymidine incorporation and by staining of cellular proteins with crystal violet. Response of fibroblasts was optimal at cell numbers of 4,000 to 9,000 cells/culture and an incubation period of four days. Serum content of the culture medium, ranging from 1 to 10% fetal calf serum (FCS), enhanced the proliferative response in a concentration-dependent manner, while higher concentrations of FCS did not lead to further increase. Both detection methods were equally suitable for the measurement of IL 1 biological activity in purified and crude preparations. In contrast to the conventional thymocyte comitogenic assay, the fibroblasts in this assay did not proliferate in response to IL 2 or IL 6. Fibroblasts were weakly stimulated by recombinant (rec) tumor necrosis factor (rec TNF-alpha); they did, however, not proliferate in response to mitogens, lipopolysaccharide, rec granulocyte-macrophage colony stimulating factor (rec GM-CSF), macrophage-CSF, rec interferon-gamma, insulin or transferrin. The detection of IL 1 activity by crystal violet staining of human dermal fibroblasts was easier and faster than by measurem...Continue Reading

  • References6
  • Citations13

Mentioned in this Paper

Structure of Calf of Leg
Tumor Necrosis Factor-alpha
Specimen Type - Fibroblasts
Granulocyte-Macrophage Colony-Stimulating Factor
Cattle calf (organism)
Bos taurus

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.