Detection of intracellular expression and secretion of interferon-gamma at the single-cell level after activation of human T cells with tetanus toxoid in vitro

European Journal of Immunology
L KabilanM Troye-Blomberg

Abstract

Activation of T cells results in intracellular expression and secretion of cytokines such as interferon (IFN)-gamma. Here we have used three different assays for determination of IFN-gamma in tetanus toxoid- or mitogen-activated human T cell cultures. Two of these assays [intracytoplasmic immunofluorescence and enzyme-linked immuno spot assay (ELISPOT)] determined the expression and secretion of IFN-gamma at the single-cell level while the third assay enzyme-linked immunosorbent assay (ELISA) measured IFN-gamma secreted into the culture supernatant. Comparison of all three tests revealed a good correlation between the ELISPOT assay and the ELISA, whereas expression of intracellular IFN-gamma showed a qualitative but not a quantitative correlation with the latter. Both the immunospot assay and the immunofluorescence may be used to detect approximate numbers of specific T cells even when present at low frequencies. With the use of the immunospot assay antigen-specific T cells could be detected even in the absence of detectable IFN-gamma in the culture supernatants. However, the ELISA assay should be more convenient for screening large clinical material.

References

Sep 1, 1989·European Journal of Immunology·B J SkidmoreJ M Chiller
Jun 1, 1987·Immunological Reviews·S SandvigU Andersson
Dec 1, 1988·European Journal of Immunology·U AnderssonG Möller
Mar 1, 1987·European Journal of Immunology·P HutchingsA Cooke
May 23, 1985·Journal of Immunological Methods·S A Möller, C A Borrebaeck
Nov 1, 1984·Scandinavian Journal of Immunology·U AnderssonS Modrow
Jan 1, 1984·Clinical Immunology and Immunopathology·P G HoltK J Turner

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Citations

Feb 26, 1993·Journal of Immunological Methods·T JungC Rieger
Nov 1, 1992·Journal of the Neurological Sciences·G SoldersT Olsson
Jul 1, 1993·Journal of the Neurological Sciences·T OlssonH Link
Nov 13, 1996·Journal of Immunological Methods·A I Dongari-Bagtzoglou, J L Ebersole
Mar 31, 1998·Journal of Immunological Methods·N KruseP Rieckmann
Nov 11, 1999·Journal of Immunological Methods·H TayebiE Robinet
Dec 10, 1999·Veterinary Immunology and Immunopathology·C M LeuteneggerH Lutz
Apr 1, 1997·Journal of Neuroimmunology·R AhlbergA K Lefvert
Jun 11, 1999·Clinical and Experimental Immunology·O JobeH Whittle
Sep 30, 2000·Clinical and Experimental Immunology·A LenarczykB Kirkham
Mar 8, 2002·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·Tomas OlssonL Arfors
Feb 1, 1994·AIDS Research and Human Retroviruses·F LolliB Wahren
Aug 1, 1995·Parasite Immunology·M R GoodierJ Langhorne
Sep 1, 1992·Scandinavian Journal of Immunology·H LinkT Olsson
Sep 1, 1992·Scandinavian Journal of Immunology·R AhlbergA K Lefvert
Nov 1, 2001·Clinical and Diagnostic Laboratory Immunology·J JasonW R Jarvis
Jun 1, 1991·The Journal of Clinical Investigation·H LinkT Olsson
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Volker LennerzThomas Wölfel
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·W HerrW J Storkus
Dec 31, 1995·Journal of Neuroimmunology·A WeishauptK V Toyka

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