PMID: 2491755Jan 1, 1989Paper

Detection of intracytoplasmic immunoglobulin by flow cytometry in B-cell malignancies

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
R H BardalesB Koziner

Abstract

The aim of this study was to compare the results of flow cytometric (FCM) determination of heavy and light chain cytoplasmic immunoglobulin (cIg) with those obtained by the peroxidase-antiperoxidase (PAP) method. Fifty-one patients, including five non-T-acute lymphoblastic leukemias, 16 B-chronic lymphocytic leukemias (CLL), 13 non-Hodgkin's lymphomas, seven hairy cell leukemias, four multiple myeloma/plasma cell leukemias, and six T-cell leukemia/lymphomas, as well as 12 normal controls, were studied. Saponin-permeabilized cell suspensions were indirectly stained with monoclonal antibodies and analyzed by flow cytometry. Acetone-fixed cytocentrifuge smears were stained for cIg by the PAP method. The results obtained indicate that: (a) detection of cIg by FCM is a feasible and useful technique to confirm the B-cell lineage of leukemias and lymphomas, particularly those characterized by low-density surface immunoglobulin, such as CLL; and (b) cIg detection by FCM and PAP staining are complementary methods to recognize with certainty the monoclonality of B-cell malignancies.

Citations

Aug 1, 1992·American Journal of Hematology·A al-KatibB Koziner
Sep 1, 1992·Leukemia Research·J OertelD Huhn
Jul 4, 2002·Hematology/oncology Clinics of North America·Roger S RileyGina Romagnoli
Oct 13, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·C C ChangD H Vesole
Nov 19, 2011·American Journal of Clinical Pathology·Pedro HornaAlexandra M Harrington
Aug 18, 2020·Cytometry. Part B, Clinical Cytometry·Agathe DebliquisBernard Drenou
Oct 31, 2020·Biosensors·Pietro SalvoTommaso Lomonaco
Oct 29, 2021·Journal of Clinical and Experimental Hematopathology : JCEH·Yosuke NakayaTakahisa Yamane

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.