Detection of intraocular Leptospira spp. by real-time polymerase chain reaction in horses with recurrent uveitis in Belgium

Equine Veterinary Journal
A C SauvageM F Grauwels

Abstract

Equine recurrent uveitis (ERU) has been associated with Leptospira spp. infection. No information exists concerning the prevalence of Leptospira-associated ERU in Belgium and about the sensitivity of detection of Leptospira in different ocular media. To establish the prevalence of intraocular Leptospira spp. in ERU-affected and healthy eyes of horses examined at the Equine Clinic of the University of Liège by real-time PCR and to compare the results of the aqueous and vitreous humour of the same eye. Cross-sectional. Sixty-six eyes from 59 client-owned horses with a diagnosis of equine recurrent uveitis (ERU-group) were studied from May 2015 to December 2017. Fifty healthy eyes from 28 euthanised horses for unrelated reasons examined during the same period were included in the control group. Intraocular fluids (aqueous and/or vitreous humours) from ERU-affected eyes were sampled and analysed by real-time PCR for Leptospira spp. Aqueous and vitreous humours from the control group were processed in the same way. Both groups were comparable regarding age, sex, eye sampled (OS/OD), humours sampled (aqueous/vitreous humour) but not regarding breeds, with an over-representation of Warmbloods and Appaloosas in the ERU-group. The preva...Continue Reading

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Citations

Apr 6, 2019·Equine Veterinary Journal·F Malalana
Dec 28, 2019·Animals : an Open Access Journal From MDPI·Elena VeraFausto Quintavalla
Oct 11, 2019·The Veterinary Record·Fernando MalalanaCathy M McGowan
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Dec 18, 2020·Letters in Applied Microbiology·M I N Di Azevedo, W Lilenbaum
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Alina CequierLaura Barrachina

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