Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells

Methods in Molecular Biology
Shun-ichi Yamashita, Tomotake Kanki

Abstract

Mitochondrial autophagy or mitophagy is a process that selectively degrades mitochondria via autophagy. It is believed that mitophagy degrades damaged or unnecessary mitochondria and is important for maintaining mitochondrial homeostasis. To date, it is known that several stimuli can induce mitophagy. However, some of these stimuli (including iron depletion, hypoxia, and nitrogen starvation) induce mild mitophagy, which is difficult to detect by measuring the decrease in mitochondrial mass. Recently, we have successfully detected mitophagy induced under these conditions using mito-Keima as a reporter. In this chapter, we describe the protocols for induction and detection of iron depletion- and hypoxia-induced mitophagy using the mito-Keima-expressing cells.

Citations

Aug 31, 2019·Investigative Ophthalmology & Visual Science·Kseniia ChernyshovaTomotake Kanki
May 3, 2021·Journal of Cellular Physiology·Shun-Ichi YamashitaTomotake Kanki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Methods in Molecular Biology
Kentaro Furukawa, Tomotake Kanki
Methods in Molecular Biology
Tomotake Kanki, Koji Okamoto
Seikagaku. The Journal of Japanese Biochemical Society
Yuko HirotaTomotake Kanki
© 2022 Meta ULC. All rights reserved