Detection of isoniazid and rifampin resistance of Mycobacteriumtuberculosis by a multiplex allele-specific polymerase chain reaction (PCR) assay

International Journal of Mycobacteriology
Zeyneb AlleguiLeila Slim-Saidi

Abstract

The use of molecular techniques is a major improvement for the rapid routine detection and control of multidrug-resistant tuberculosis (MDR-TB). In this study, the multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to simultaneously detect the most frequent mutations associated with isoniazid (INH) and rifampin (RIF) resistance in a single assay. The assay was tested with 53 clinical isolates. Among them, 27 were MDR strains, 17 were mono-resistant to INH, one was mono-resistant to RIF, and eight were susceptible. The MAS-PCR assay showed a specificity of 100% in detecting drug resistance. An equivalent sensitivity of 92.6% in detecting MDR and RIF-resistance was found. The sensitivity for the detection of INH-resistance was 88.6%. The MAS-PCR assay was a simple and rapid method for detecting the INH and RIF-resistance in Mycobacterium tuberculosis (MT) clinical strains. It is also easy to perform and to interpret. The assay is inexpensive and a less-demanding technique.

References

Oct 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·H De BeenhouwerF Portaels
Mar 1, 1996·Molecular Microbiology·T M Wilson, D M Collins
Mar 13, 1999·Journal of Clinical Microbiology·G PozziG Orefici
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Srinivas V RamaswamyEdward A Graviss
Jun 25, 2003·Antimicrobial Agents and Chemotherapy·Igor MokrousovOlga Narvskaya
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Laura Herrera-LeónMaria Soledad Jiménez
Jul 28, 2006·Antimicrobial Agents and Chemotherapy·Manzour Hernando HazbónDavid Alland
Sep 21, 2010·Pathologie-biologie·A GravetJ-M Delarbre

❮ Previous
Next ❯

Citations

Nov 17, 2016·International Journal of Mycobacteriology·Mohanad M AhmedSuhad H Mohammed
Oct 14, 2021·World Journal of Microbiology & Biotechnology·Ei Phoo ThweArunnee Sangka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.