Detection of Janus Kinase 2 gene single point mutation in real samples with electrochemical DNA biosensor

Clinica Chimica Acta; International Journal of Clinical Chemistry
Seda Nur TopkayaMehmet Ozsoz

Abstract

Janus Kinase 2 (JAK2) gene single point mutations, which have been reported to be associated with myeloproliferative disorders, are usually detected through conventional methods such as melting curve assays, allele-specific and quantitative Polymerase Chain Reactions (PCRs). Herein, an electrochemical biosensor for the detection of a Guanine (G) to Thymine (T) transversion at nucleotide position 1849 of the JAK2 gene was reported. Due to clinical importance of this mutation, easy and sensitive tests are needed to be developed. Our aim was to design a biosensor system that is capable of detecting the mutation within less than 1h with high sensitivity. For these purposes, an electrochemical sensing system was developed based on detecting hybridization. Hybridization between probe and its target and discrimination of single point mutation was investigated by monitoring guanine oxidation signals observed at +1.0 V with Differential Pulse Voltammetry (DPV) by using synthetic oligonucleotides and Polymerase Chain Reaction (PCR) amplicons. Hybridization between probe and PCR amplicons was also determined with Electrochemical Impedance Spectroscopy (EIS). We successfully detect hybridization first in synthetic samples, and ultimately i...Continue Reading

References

Sep 5, 2006·Biosensors & Bioelectronics·Patricia Abad-ValleAgustín Costa-García
Feb 23, 2008·Trends in Biochemical Sciences·Stefan N ConstantinescuChristian Pecquet
Jul 15, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tze-Kiong ErTa-Chih Liu
Aug 24, 2010·Biosensors & Bioelectronics·Pinar KaraArben Merkoçi
Oct 12, 2010·Experimental Hematology·Kausar Begam Riaz AhmedCharles J Parker

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Citations

Oct 30, 2014·Analytical Chemistry·Chengzhou ZhuYuehe Lin
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley

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