Abstract
Janus Kinase 2 (JAK2) gene single point mutations, which have been reported to be associated with myeloproliferative disorders, are usually detected through conventional methods such as melting curve assays, allele-specific and quantitative Polymerase Chain Reactions (PCRs). Herein, an electrochemical biosensor for the detection of a Guanine (G) to Thymine (T) transversion at nucleotide position 1849 of the JAK2 gene was reported. Due to clinical importance of this mutation, easy and sensitive tests are needed to be developed. Our aim was to design a biosensor system that is capable of detecting the mutation within less than 1h with high sensitivity. For these purposes, an electrochemical sensing system was developed based on detecting hybridization. Hybridization between probe and its target and discrimination of single point mutation was investigated by monitoring guanine oxidation signals observed at +1.0 V with Differential Pulse Voltammetry (DPV) by using synthetic oligonucleotides and Polymerase Chain Reaction (PCR) amplicons. Hybridization between probe and PCR amplicons was also determined with Electrochemical Impedance Spectroscopy (EIS). We successfully detect hybridization first in synthetic samples, and ultimately i...Continue Reading
References
Apr 26, 2001·Analytical Chemistry·K M HansenT Thundat
Mar 29, 2005·Nature·Chloé JamesWilliam Vainchenker
Apr 20, 2005·Cancer Cell·Ross L LevineD Gary Gilliland
Nov 5, 2005·Leukemia·R McClureT Lasho
Dec 13, 2005·Leukemia·C JamesN Casadevall
Dec 15, 2005·Blood·Amy V JonesNicholas C P Cross
May 6, 2006·Blood·Robert KralovicsRadek C Skoda
Sep 5, 2006·Biosensors & Bioelectronics·Patricia Abad-ValleAgustín Costa-García
Jun 2, 2007·Leukemia·A PardananiA Tefferi
Nov 7, 2007·Blood·Elizabeth O HexnerPawel Dobrzanski
Feb 5, 2008·Analytical Biochemistry·Ali MehdiniaMir Fazlollah Mousavi
Feb 23, 2008·Trends in Biochemical Sciences·Stefan N ConstantinescuChristian Pecquet
Jul 15, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tze-Kiong ErTa-Chih Liu
Aug 24, 2010·Biosensors & Bioelectronics·Pinar KaraArben Merkoçi
Oct 12, 2010·Analytical Chemistry·Roya KalantariMira Josowicz
Oct 12, 2010·Experimental Hematology·Kausar Begam Riaz AhmedCharles J Parker
Dec 2, 2010·International Journal of Laboratory Hematology·G C ShepardJ Owen
Mar 19, 2013·Chemical Communications : Chem Comm·Yao Wu, Rebecca Y Lai
Citations
Oct 30, 2014·Analytical Chemistry·Chengzhou ZhuYuehe Lin
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Apr 1, 2020·Analytical Chemistry·Yuan LiuYuling Wang