Detection of JC virus-specific cytotoxic T lymphocytes in healthy individuals

Journal of Virology
R A Du PasquierI J Koralnik

Abstract

The polyomavirus JC (JCV) infects 85% of healthy individuals, and its reactivation in a limited number of immunosuppressed people causes progressive multifocal leukoencephalopathy (PML), a severe demyelinating disease of the central nervous system. We hypothesized that JCV-specific cytotoxic T lymphocytes (CTLs) might control JCV replication in healthy individuals, blocking the evolution of PML. Using 51Cr release and tetramer staining assays, we show that 8 of 11 HLA-A*0201+ healthy subjects (73%) harbor detectable JCV-specific CD8+ CTLs that recognize one or two epitopes of JCV VP1 protein, the HLA-A*0201-restricted VP1p36 and VPp1100 epitopes. We determined that the frequency of JCV VP1 epitope-specific CTLs varied from less than 1/100,000 to 1/2,494 peripheral blood mononuclear cells. More individuals had JCV VP1-specific than cytomegalovirus-specific CTLs (8 of 11 subjects [73%] versus 2 of 10 subjects [20%], respectively). These results show that a CD8+-T-cell response against JCV is commonly found in immunocompetent people and suggest that these cells might protect against the development of PML.

References

Jan 1, 1985·Immunogenetics·R D SalterP Cresswell
Nov 6, 1965·British Medical Journal·E Klemola, L Kääriäinen
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D CatalinaK Luzuriaga
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Igor J KoralnikNorman L Letvin

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Citations

Apr 9, 2005·Nature Biotechnology·Cormac Sheridan
May 21, 2005·Nature Reviews. Drug Discovery·Cormac Sheridan
Jun 11, 2005·The New England Journal of Medicine·Joseph R Berger, Igor J Koralnik
Nov 4, 2005·The New England Journal of Medicine·Daniel K Podolsky
May 3, 2012·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Chen Sabrina TanIgor J Koralnik
Dec 6, 2011·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M A SahraianA Minagar
Dec 13, 2012·Journal of Translational Medicine·Roberta MancusoMario Clerici
Sep 6, 2014·Revue neurologique·M Spatola, R A Du Pasquier
Dec 4, 2013·Journal of Neurovirology·Sarah Beltrami, Jennifer Gordon
May 7, 2015·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yukiko Shishido-Hara
Sep 12, 2015·European Journal of Immunology·Guillaume Martin-BlondelRoland Liblau
Jul 29, 2008·Neurologic Clinics·Thomas Weber
Oct 13, 2010·Journal of Neuroimmunology·Robert Weissert
Sep 26, 2009·The Lancet Infectious Diseases·Paola CinqueRichard W Price
Dec 15, 2015·Reviews in Medical Virology·Martyn K WhiteKamel Khalili
Mar 10, 2016·Journal of Neurovirology·Rahsan SariyerIlker Kudret Sariyer
Jul 3, 2007·Clinical Neurology and Neurosurgery·Hans-Ullrich VölkerStephanie Sommer
Apr 9, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Clinton D CrowderEmilio Ramos
May 12, 2007·Brain Pathology·Ikuo TsunodaRobert S Fujinami
Jul 11, 2006·Immunological Reviews·Alexandre HarariGiuseppe Pantaleo
Sep 27, 2008·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·Sajeel Chowdhary, Marc Chamberlain
Aug 20, 2010·Arthritis and Rheumatism·Deepak KumarRobert G Berger
Jun 21, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hans H HirschCeline Leboeuf
Jun 6, 2006·Journal of Neuroimmunology·Renaud A Du PasquierIgor J Koralnik
Jul 8, 2015·Annals of Neurology·Thorsten BartschIgor J Koralnik
Feb 14, 2015·Medical Microbiology and Immunology·Jens VerheyenAndrea Rubbert-Roth
Apr 1, 2014·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Chen Sabrina TanIgor J Koralnik
Jul 20, 2006·Expert Opinion on Therapeutic Targets·Yi-Yang Yvonne LiThomas M Zollner
Jan 16, 2010·MAbs·Joseph R BergerEugene O Major

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