PMID: 2481639Nov 1, 1989Paper

[Detection of keratin mRNA and human origin of xenotransplanted human tracheobronchial epithelium by in situ hybridization].

[Hokkaido igaku zasshi] The Hokkaido journal of medical science
T Obara

Abstract

An in situ hybridization method was applied to detect keratin mRNA expression and human origin of xenotransplanted human tracheobronchial epithelium into nude mice. Tissues from eight tracheas repopulated with cells from five different donors were used. A K6 (56 kd) human keratin cDNA (KA-1) and a K14 (50 kd) cDNA (KB-2) were radiolabeled with 3H-dATP/TTP and used as probes to study changes in keratin mRNA expression in various lesions of the tracheobronchial epithelium. To identify human tissues xenotransplanted into nude mice, high-molecular-weight DNAs extracted from human or mouse tissues were sonicated, nick-translated with 32p-dCTP, and used as probes. Dot-blot hybridization of 32p-labeled DNA probes revealed clear species-specific signals and cells of human origin were distinguishable by in situ hybridization with sonicated human DNA probe. In situ hybridization with either KA-1 or KB-2 probe showed similar localization of silver grains in all histologic types in consecutive tissue lesions of xenotransplanted tracheobronchial epithelia. Very few grains were seen over cells of simple, pseudostratified, or stratified epithelia two to three cell layer thick. Nonkeratinizing stratified hyperplastic epithelia of more than thr...Continue Reading

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