Detection of large plasmids from the Bacillus cereus group

Plasmid
Lars AndrupLasse Smidt

Abstract

The members of the Bacillus cereus group, Bacillus anthracis, Bacillus thuringiensis, and B. cereus senso stricto, are largely defined by their content of large plasmids, which encode major virulence factors. Here we offer an easy, fast, and reliable protocol for the isolation and detection of large plasmids up to the size of at least 350kb. Furthermore, using this method, we report that Bacillus mycoides contain large plasmids.

References

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Citations

Jun 1, 2009·Environmental Microbiology Reports·Florence M HotonJacques Mahillon
Oct 30, 2016·Research in Microbiology·Annika GillisJacques Mahillon
Nov 5, 2016·Research in Microbiology·Alexandre BolotinAlexei Sorokin
Jun 25, 2019·Journal of Food Protection·Klèma Marcel KonéJacques Mahillon
Dec 15, 2020·Applied and Environmental Microbiology·Nancy FayadMireille Kallassy Awad

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