Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

Transactions of the Royal Society of Tropical Medicine and Hygiene
S AdhyaS Sen

Abstract

Samples from 39 patients with symptoms suggestive of early visceral leishmaniasis were independently assayed by microscopy of tissue smears, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) of blood deoxyribonucleic acid. Of these patients, 19 were confirmed as positive or negative by all 3 tests; 11 patients (28%) negative by smear were positive by ELISA and PCR; and 7 (18%) were positive by PCR alone. These results demonstrate the high sensitivity of the non-invasive PCR and, to a smaller extent, ELISA, in the early diagnosis of visceral leishmaniasis.

References

Jun 1, 1978·Annals of Tropical Medicine and Parasitology·M HommelG Lanotte
Mar 1, 1975·The American Journal of Tropical Medicine and Hygiene·D S Allain, I G Kagan
Jan 1, 1990·Medical and Veterinary Entomology·R Killick-Kendrick
Oct 1, 1990·Experimental Parasitology·M R RodgersD F Wirth
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·C L Jaffe, D McMahon-Pratt

❮ Previous
Next ❯

Citations

Nov 20, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·Simonne De DonckerJean-Claude Dujardin
Sep 25, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sunil K AroraN L Sharma
Nov 16, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Pankaj SrivastavaShyam Sundar
Jan 2, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·M BoelaertP Van der Stuyft
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sergio G CoutinhoAlda Maria Da-Cruz
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Samit AdhyaSantu Bandyopadhyay
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·I CruzUNKNOWN Spanish HIV-Leishmania Study Group
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·R FisaM Portús
Jul 21, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·J DischA Rabello
Sep 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·G ChakrabartiS Bandyopadhyay
Dec 10, 1999·Tropical Medicine & International Health : TM & IH·M Boelaert, J C Dujardin
Aug 9, 2002·Tropical Medicine & International Health : TM & IH·Henk D F H Schallig, Linda Oskam
Jan 5, 2002·Journal of Clinical Microbiology·Laurence LachaudPatrick Bastien
Jul 8, 2005·Journal of Clinical Microbiology·R MauryaS Sundar
Mar 24, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Jamshid Kohanteb, Sadreddin Ardehali
May 12, 2011·PloS One·Pankaj SrivastavaShyam Sundar
Jul 16, 2014·Diagnostic Microbiology and Infectious Disease·Medhavi Sudarshan, Shyam Sundar
Apr 9, 2013·Revista Do Instituto De Medicina Tropical De São Paulo·Luciana Almeida SilvaAluízio Prata
Jun 1, 2010·Memórias do Instituto Oswaldo Cruz·Thiago Leite FragaClaude Pirmez
Jan 29, 2002·Revista Da Sociedade Brasileira De Medicina Tropical·D B LiarteC H Costa
Oct 13, 2007·Diagnostic Microbiology and Infectious Disease·Motahareh MotazedianFataneh Mikaeili
Sep 3, 2002·Clinical and Diagnostic Laboratory Immunology·Shyam Sundar, M Rai
Jun 21, 2018·Molecular Diagnosis & Therapy·Shyam Sundar, Om Prakash Singh
Mar 23, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Wellington Francisco RodriguesCamila Botelho Miguel
Apr 1, 1997·Clinical Microbiology Reviews·J AlvarJ Moreno
Jul 3, 1999·Clinical and Diagnostic Laboratory Immunology·M ChatterjeeC Mandal
Dec 20, 2002·Molecular and Cellular Probes·Gregory SpanakosNicholas Vakalis
Oct 8, 1997·Journal of Clinical Microbiology·O F OsmanP A Kager

❮ Previous
Next ❯

Related Concepts