Detection of lymph node micrometastases by gene promoter hypermethylation in samples obtained by endosonography- guided fine-needle aspiration biopsy

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Maria PelliséJosep M Piqué

Abstract

Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) has become a fundamental procedure for gastrointestinal and lung cancer staging. However, there is growing evidence that micrometastases are present in lymph nodes, which cannot be detected with standard pathological methods. The aim of this study was to evaluate whether hypermethylation gene promoter analysis was feasible on samples obtained by EUS-FNA from lymph nodes, as well as to establish the usefulness of this strategy for the detection of micrometastases in patients with gastrointestinal and non-small cell lung cancer. Suspicious lymph nodes based on EUS findings from consecutive patients with esophageal, gastric, rectal, and non-small cell lung cancer were sampled by EUS-FNA. Hypermethylation analysis of the MGMT, p16(INK4a), and p14(ARF) gene promoter CpG islands were performed by methylation-specific PCR. Effectiveness of conventional cytology, methylation analysis, and their combination were established with respect to the definitive diagnosis. Twenty-seven patients were included, thus representing a total of 42 lymph nodes (esophageal cancer, n = 11; rectal cancer, n = 7; gastric cancer, n = 3; and lung cancer, n = 21). According to definitive diagnosis,...Continue Reading

References

Jan 1, 1995·The Journal of Thoracic and Cardiovascular Surgery·N MartiniR J Ginsberg
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Oct 24, 1997·The Journal of Thoracic and Cardiovascular Surgery·R MaruyamaK Sugimachi
Mar 18, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A CastellsA Farré
May 26, 2001·Gastrointestinal Endoscopy·E Vazquez-SequeirosM J Wiersema
Jun 23, 2001·Annals of Surgery·S A WajedT R DeMeester
Feb 28, 2002·The American Journal of Gastroenterology·Gavin C Harewood, Maurits J Wiersema
Mar 8, 2002·The Journal of Thoracic and Cardiovascular Surgery·Steven A AhrendtDavid Sidransky
Mar 5, 2003·American Journal of Respiratory and Critical Care Medicine·Michael B WallaceAlice Boylan
Jun 6, 2003·The Lancet Oncology·Manel Esteller
Jul 3, 2003·Journal of the National Cancer Institute·Steven A AhrendtDavid Sidransky

❮ Previous
Next ❯

Citations

Jan 12, 2005·The Journal of Pathology·Manel Esteller
Nov 23, 2006·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·S GretschelP M Schlag
Dec 3, 2005·Journal of Gastroenterology·Kenji YamaoTakashi Koshikawa
Apr 4, 2008·Clinical & Experimental Metastasis·David I Rodenhiser
Mar 14, 2008·The New England Journal of Medicine·Malcolm V BrockJames G Herman
Sep 13, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·K DarwicheL Freitag
Feb 6, 2009·Nuclear Medicine Communications·Ali SalavatiAbass Alavi
Feb 18, 2009·Journal of Gastroenterology and Hepatology·Kenji YamaoAtsushi Irisawa
Feb 15, 2007·Respirology : Official Journal of the Asian Pacific Society of Respirology·Kazuhiro Yasufuku, Takehiko Fujisawa
May 22, 2008·World Journal of Gastroenterology : WJG·Srinivas-R PuliMainor-R Antillon
Jan 19, 2010·World Journal of Gastroenterology : WJG·Masatsugu HirakiKohji Miyazaki
Jan 7, 2014·Archivos de bronconeumología·Virginia Leiro-FernándezAlberto Fernández-Villar
Dec 18, 2004·Critical Reviews in Clinical Laboratory Sciences·Mukesh VermaSudhir Srivastava
Mar 24, 2015·Expert Review of Molecular Diagnostics·Diego M Marzese, Dave Sb Hoon
Jul 15, 2005·Expert Review of Molecular Diagnostics·Surakit PungpapongMichael B Wallace
May 13, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Ana Belén CustodioEduardo Díaz-Rubio
Aug 10, 2011·Archivos de bronconeumología·Julio Sánchez de CosUNKNOWN Sociedad Española Neumología y Cirugía Torácica
Sep 12, 2009·Best Practice & Research. Clinical Gastroenterology·M Polkowski
Feb 21, 2009·Gastrointestinal Endoscopy·Michael B Wallace
Mar 4, 2008·Gastroenterology Clinics of North America·Manoop S Bhutani
Mar 3, 2006·Diagnostic Cytopathology·Ricardo H BardalesMichael W Stanley
May 13, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·P Fusaroli, G Caletti
Dec 6, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Louis BuscailJean Escourrou
Sep 27, 2005·The Annals of Thoracic Surgery·Malcolm V BrockJames G Herman
Jun 30, 2015·Clinical & Experimental Metastasis·Laura MillaresEduard Monsó
Jan 24, 2012·Gynecologic Oncology·M H M OonkA G J van der Zee
Jul 9, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Charles J Lightdale, Ketan G Kulkarni
Jan 1, 2011·Cancers·Barbara BournetPierre Cordelier
Jan 1, 2009·Journal of Bronchology & Interventional Pulmonology·Takahiro NakajimaIchiro Yoshino
Apr 6, 2005·Human Molecular Genetics·Peter W Laird
Jul 16, 2008·Current Opinion in Gastroenterology·Laith H JamilMichael B Wallace
Oct 24, 2014·World Journal of Gastroenterology : WJG·Mario TadicRajko Kusec
Mar 8, 2006·Archives of Pathology & Laboratory Medicine·Alberto M Marchevsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.