Detection of mammalian reovirus RNA by using reverse transcription-PCR: sequence diversity within the lambda3-encoding L1 gene.

Journal of Clinical Microbiology
Thomas P LearyT S Dermody

Abstract

Reoviruses infect virtually all mammalian species, and infection of humans is associated with mild gastrointestinal or upper respiratory illnesses. To improve reovirus detection strategies, we developed a reverse transcription-PCR technique to amplify a fragment of the reovirus L1 gene segment. This assay was capable of detecting 44 of 44 reovirus field isolate strains and was sufficiently sensitive to detect nearly a single viral particle (1.16 +/- 0.13) per PCR of prototype strain type 3 Dearing. Pairwise comparisons of the 44 partial L1 gene sequences revealed that nucleotide variability ranged from 0 to 24.7%, with most of the nucleotide polymorphism occurring at synonymous positions. Phylogenetic trees generated from amplified L1 gene sequences suggest that multiple alleles of the L1 gene cocirculate in nature and that genetic diversity of the L1 gene is largely independent of the host species, geographic locale, or date of isolation. Phylogenetic trees constructed from the L1 gene sequences are distinct from those constructed from the four reovirus S-class gene segments, which supports the hypothesis that reovirus gene segments reassort in nature. This study establishes a new sensitive and specific technique for the ident...Continue Reading

References

Jul 1, 1992·Journal of Pediatric Surgery·K ParasharM A McCrae
Feb 1, 1989·Applied and Environmental Microbiology·D R DahlingB A Wright
Nov 1, 1985·Journal of Virology·E A WenskeB N Fields
Oct 1, 1973·The Journal of General Virology·M F MillerL Dmochowski
May 1, 1967·British Medical Bulletin·N F Stanley
Jan 1, 1982·Journal of Virology·D Drayna, B N Fields
Sep 1, 1982·Applied and Environmental Microbiology·D J AdamsB B Barnett
Mar 1, 1993·Virology·M C Starnes, W K Joklik
Jun 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K L TylerW R Brown
Dec 1, 1954·Proceedings of the Society for Experimental Biology and Medicine·M RAMOS-ALVAREZ, A B SABIN
May 10, 1958·Journal of the American Medical Association·M RAMOS-ALVAREZ, A B SABIN
Nov 8, 1962·The New England Journal of Medicine·A M LERNERM FINLAND

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Citations

Oct 13, 2006·Annals of Biomedical Engineering·Gregory P StoneFrederick R Haselton
Jun 10, 2015·Viruses·Robert HollingworthRoger J Grand
Nov 25, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·N DecaroC Buonavoglia
Oct 17, 2014·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Walter Q BetancourtCharles P Gerba
Jun 20, 2016·Food and Environmental Virology·Walter Q Betancourt, Charles P Gerba
Dec 1, 2015·The Journal of Veterinary Medical Science·Shinobu TsuchiakaTetsuya Mizutani
Feb 6, 2007·Virus Research·Nicola DecaroCanio Buonavoglia
Dec 9, 2010·International Journal of Environmental Research and Public Health·Elvira TarsitanoMaria Tempesta
Oct 27, 2012·Journal of Clinical Microbiology·Nicola DecaroCanio Buonavoglia
Feb 14, 2007·Veterinary Research·Canio Buonavoglia, Vito Martella
Apr 23, 2020·Animals : an Open Access Journal From MDPI·Camilla LuzzagoStefania Lauzi
Aug 31, 2012·Zoonoses and Public Health·D LelliP Cordioli
May 28, 2020·Viruses·Lara CavicchioMaria Serena Beato
Sep 5, 2018·BMC Veterinary Research·Tina NagličAndrej Steyer
Aug 7, 2021·Transboundary and Emerging Diseases·Chutchai PiewbangSomporn Techangamsuwan

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