Detection of maternal DNA in umbilical cord blood by polymerase chain reaction amplification of minisatellite sequences

Bone Marrow Transplantation
M BrizM N Fernández

Abstract

One of the concerns about the use of cord blood as a source of hematopoietic stem cells for allogeneic transplantation is the possibility of contamination by maternal cells which could cause life-threatening GVHD. We have assessed cord blood contamination using PCR analysis of several minisatellite regions to detect maternal DNA. Eighty mother-cord pairs were obtained for this study. In one case there were no specific maternal alleles at any loci and, therefore, cord blood could not be evaluated. Thus, there was a total of 79 informative cases for the detection of maternal cells in the fetal circulation. In most cases, the level of detection was between 0.5 and 1%. We detected maternal DNA in the cord blood sample in only one case (1.26%), and the analysis of dilution experiments led to an estimate of 0.5-1% maternal cells. In conclusion, using PCR amplification of hypervariable regions, maternal DNA is very rarely detected in the cord blood collected at birth, although this approach has a relatively low level of sensitivity.

Citations

Sep 11, 2002·The Journal of Gene Medicine·Makoto HiranoTakahisa Imamura
Mar 17, 2004·Atherosclerosis·Olivier S DescampsFrancis R Heller
Jun 7, 2007·Pediatric Rheumatology Online Journal·Anne M Stevens
Jan 10, 2003·The Journal of Pediatrics·Bharath SrivatsaDiana W Bianchi
Jun 28, 2018·Cell Transplantation·Anna Maria Jonsson KanoldCecilia Götherström
Mar 29, 2019·Scientific Reports·Rianne OpsteltenDerk Amsen
Jul 27, 2001·British Journal of Haematology·A F Eder, C S Manno
May 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Annette M MarleauB Anne Croy

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