Detection of mcr-1 Plasmids in Enterobacteriaceae Isolates From Human Specimens: Comparison With Those in Escherichia coli Isolates From Livestock in Korea

Annals of Laboratory Medicine
Eun Jeong YoonSeok Hoon Jeong

Abstract

The emerging mobile colistin resistance gene, mcr-1, is an ongoing worldwide concern and an evaluation of clinical isolates harboring this gene is required in Korea. We investigated mcr-1-possessing Enterobacteriaceae among Enterobacteriaceae strains isolated in Korea, and compared the genetic details of the plasmids with those in Escherichia coli isolates from livestock. Among 9,396 Enterobacteriaceae clinical isolates collected between 2010 and 2015, 1,347 (14.3%) strains were resistant to colistin and those were screened for mcr-1 by PCR. Colistin minimum inhibitory concentrations (MICs) were determined by microdilution, and conjugal transfer of the mcr-1-harboring plasmids was assessed by direct mating. Whole genomes of three mcr-1-positive Enterobacteriaceae clinical isolates and 11 livestock-origin mcr-1-positive E. coli isolates were sequenced. Two E. coli and one Enterobacter aerogenes clinical isolates carried carried IncI2 plasmids harboring mcr-1, which conferred colistin resistance (E. coli MIC, 4 mg/L; E. aerogenes MIC, 32 mg/L). The strains possessed the complete conjugal machinery except for E. aerogenes harboring a truncated prepilin peptidase. The E. coli plasmid transferred more efficiently to E. coli than to ...Continue Reading

References

Aug 1, 1982·Journal of General Microbiology·D E Bradley, J N Coetzee
Mar 27, 1999·Journal of Bacteriology·T YoshidaT Komano
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Sep 15, 2009·Drugs·Helen Giamarellou, Garyphallia Poulakou
Dec 17, 2010·The New England Journal of Medicine·Robert C Moellering
Feb 5, 2016·The Lancet Infectious Diseases·Hong DuBarry N Kreiswirth
Mar 10, 2016·Antimicrobial Agents and Chemotherapy·Shimaa S ElnahriryTadashi Shimamoto
Mar 12, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Robert L Skov, Dominique L Monnet
Mar 15, 2016·The Lancet Infectious Diseases·Zhangqi ShenCongming Wu
May 11, 2016·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
May 19, 2016·Emerging Infectious Diseases·Xue-Fei ZhangGuo-Bao Tian
May 25, 2016·Antimicrobial Agents and Chemotherapy·Jian SunYa-Hong Liu
Jul 16, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Basil Britto XavierSurbhi Malhotra-Kumar
Aug 1, 2016·Transactions of the Royal Society of Tropical Medicine and Hygiene·T P RobinsonM E J Woolhouse
Aug 10, 2016·Antimicrobial Agents and Chemotherapy·Jocelyn Qi-Min TeoAndrea Lay-Hoon Kwa
Aug 26, 2016·The Journal of Antimicrobial Chemotherapy·Christian J H von WintersdorffJohn Penders
Aug 31, 2016·Antimicrobial Agents and Chemotherapy·Suk-Kyung LimSuk-Chan Jung
Nov 29, 2016·PLoS Pathogens·Rongsui GaoYoujun Feng
Feb 23, 2017·Antimicrobial Agents and Chemotherapy·Erik SnesrudPatrick McGann
Aug 7, 2017·Diagnostic Microbiology and Infectious Disease·Eun Sil KimYang Soo Kim
Aug 12, 2017·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Alessandra CarattoliChiara Francesca Magistrali

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Citations

Sep 30, 2020·The Journal of Antimicrobial Chemotherapy·Cristina F MallaMaría M Tavío
Sep 23, 2020·Journal of Microbiology and Biotechnology·Seokhwan KimSangryeol Ryu
Nov 2, 2019·Infection & chemotherapy·Ji Young ParkJi Ae Choi
Aug 8, 2021·The Science of the Total Environment·Shaqiu ZhangAnchun Cheng

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Datasets Mentioned

BETA
KY657478
KY657476
KY657477

Methods Mentioned

BETA
PCR

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