PMID: 3766954Aug 15, 1986Paper

Detection of metallothionein on nitrocellulose membrane using Western blotting technique and its application to identification of cadmium-binding proteins

Analytical Biochemistry
Y AokiK T Suzuki

Abstract

A convenient method for detecting metallothionein (MT) and other cadmium-binding proteins (Cd-BPs) in tissue cytosol was established. A sample that contains MT was separated on a sodium dodecyl sulfate-polyacrylamide gel and then electrophoretically transferred to a nitrocellulose membrane after reduction with 2-mercaptoethanol (2-ME) on the gel. The membrane was incubated in a buffer containing 109Cd and then subjected to autoradiography. MT-I and -II in rat liver cytosol were detected as radioactive bands together with a Cd-BP of 60,000 Da. On the other hand, three Cd-BPs of 40,000, 29,000, and 24,000 Da were detected in the cytosol when the reduction with 2-ME was omitted.

References

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Citations

Oct 28, 2008·Applied Biochemistry and Biotechnology·Angélica María Ortiz-BuenoEduardo Brambila
Oct 1, 1991·Journal of Biochemical and Biophysical Methods·Y AokiK T Suzuki
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·O J MesnaR A Andersen
Jan 1, 1989·General Pharmacology·A MikalsenR A Andersen
Jan 1, 1989·General Pharmacology·R A AndersenJ Alexander
Jan 1, 1990·General Pharmacology·O J MesnaR A Andersen
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·R A AndersenJ Alexander
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Kunimoto, T Miura
Dec 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U I Walther, W Forth
Mar 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Z KrocovaL Hernychova
Jan 1, 1988·Critical Reviews in Food Science and Nutrition·R NathM Sharma
Mar 13, 2012·Journal of Neurochemistry·Jong-Heon KimKyoungho Suk
Apr 9, 2001·Human & Experimental Toxicology·U I WaltherW Forth
Jan 1, 1996·Biological Trace Element Research·J I Alonso, M C Martin-Mateo
Jan 1, 1989·Archives of Andrology·G FrenetteJ Y Dubé
Feb 10, 1998·International Journal of Cancer. Journal International Du Cancer·T L EraiserG I Abelev
Apr 28, 1995·Journal of Chromatography. a·P J Wirth, A Romano
Jul 1, 1988·Analytical Biochemistry·A MazenG de Murcia
Jun 1, 1991·Analytical Biochemistry·F SimoninG de Murcia
Nov 27, 1991·Biochemical and Biophysical Research Communications·T FurukawaR Tokunaga

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