Detection of methylated CDO1 in plasma of colorectal cancer; a PCR study.

PloS One
Keishi YamashitaMasahiko Watanabe

Abstract

Cysteine biology is important for the chemosensitivity of cancer cells. Our research has focused on the epigenetic silencing of cysteine dioxygenase type 1 (CDO1) in colorectal cancer (CRC). In this study, we describe detection of CDO1 methylation in the plasma of CRC patients using methylation specific PCR (Q-MSP) and extensive analysis of the PCR reaction. DNA was extracted from plasma, and analysed for methylation of the CDO1 gene using Q-MSP. The detection rate of CDO1 gene methylation was calculated and compared with that of diluted DNA extracted from "positive control" DLD1 cells. CDO1 gene methylation in the plasma of 40 CRC patients that were clinicopathologically analysed was then determined. (1) The cloned sequence analysis detected 93.3% methylation of the promoter CpG islands of the CDO1 gene of positive control DLD1 cells and 4.7% methylation of the negative control HepG2 CDO1 gene. (2) DLD1 CDO1 DNA could not be detected in this assay if the extracted DNA was diluted ∼1000 fold. The more DNA that was used for the PCR reaction, the more effectively it was amplified in Q-MSP. (3) By increasing the amount of DNA used, methylated CDO1 could be clearly detected in the plasma of 8 (20%) of the CRC patients. However, the...Continue Reading

References

Apr 1, 1980·Diseases of the Colon and Rectum·J P ArnaudM Adloff
Nov 18, 2000·Journal of the National Cancer Institute·K KawakamiS J Meltzer
Jul 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Devin DressmanBert Vogelstein
Jan 21, 2006·Cancer Research·Hiroyuki MatsubayashiMichael Goggins
Dec 20, 2007·Clinical Chemistry·Catherine Lofton-DayAndrew Sledziewski
Mar 15, 2008·World Journal of Surgery·Hiroshi KatohMasahiko Watanabe
Feb 10, 2009·Cancer Science·Keishi Yamashita, Masahiko Watanabe
May 2, 2009·Clinical Chemistry·Theo deVosCatherine Lofton-Day
Aug 18, 2009·Nature Biotechnology·Meng LiBert Vogelstein
May 1, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jana JeschkeNita Ahuja
Dec 20, 2013·Cancer Research·Antony PrabhuPrakash Chinnaiyan

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Citations

Mar 31, 2017·International Journal of Molecular Sciences·Keigo YokoiMasahiko Watanabe
Mar 1, 2017·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Hideki UshikuMasahiko Watanabe
Sep 29, 2018·Cancer Science·Keishi YamashitaMasahiko Watanabe
Sep 16, 2016·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Hideki UshikuMasahiko Watanabe
Apr 10, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Bi WangLin Huang
Feb 12, 2017·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·K KojimaM Watanabe
Aug 11, 2020·The Journal of Surgical Research·Hiroki HaradaKeishi Yamashita

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Methods Mentioned

BETA
PCR
surgical resection
chemical modification

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