Detection of microbial contamination in fermentation processes: mass spectrometric determination of gram-negative bacteria in Leuconostoc mesenteroides cultures

Biotechnology and Bioengineering
I ElmrothL Larsson


Gas chromatography/mass spectrometry was used to detect the presence of Enterobacter cloacae in cultures of Leuconostoc mesenteroides, an organism used in an industrial process for production of dextrane. The penta-fluorobenzoyl-methyl ester derivative of 3-hydroxy-myristic acid, a characteristic compound of gram-negative bacteria, was used as the analyte. By using gas chromatography/mass spectrometry with selected ion monitoring, E. cloacae was determined over the range of 1 ppm to 1% in cultures of L. mesenteroides. The proposed analytical approach represents a useful alternative to conventional methods for determining contaminating organisms in industrial fermentation processes.


Jan 1, 1977·Journal of Medical Virology·A LevanonA Kohn
Dec 1, 1985·Applied and Environmental Microbiology·J M HensonD C White
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S K MaitraL B Guze

Related Concepts

Gram-Negative Bacteria
Gas Chromatography-Mass Spectrometry
Myristic Acids
Dextran Sulfate Sodium
Enterobacter cloacae
Laboratory Culture
Detected (Finding)
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Candida cloacae

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