Detection of microcystin-producing cyanobacteria in water samples using loop-mediated isothermal amplification targeting mcyB gene

3 Biotech
Mohandass RamyaPasupathi Rathinasabapathi

Abstract

Microcystin toxin-producing cyanobacteria are known to have harmful effects on humans and animals. We have developed a loop-mediated isothermal amplification (LAMP)-based detection method by targeting the microcystin synthetase B gene (mcyB), the gene responsible for the production of microcystin. The sensitivity of the method was found to be 1 fg per reaction, and it was 1000-fold higher than the conventional PCR. The LAMP method was able to amplify the target gene with a minimum amount of dNTP (0.4 mM), which further reduces the cost of reaction. The improved LAMP assay could detect the presence of the toxin-producing cyanobacteria in water samples within 2 h of time, which demonstrates the rapidness of the method. Freshwater samples were screened using the developed LAMP, and seven water samples collected from lakes and a bird sanctuary tested positive for mcyB gene harboring cyanobacteria, and negative in all other drinking waters. Hence, the developed LAMP could be a possible alternative to the existing molecular methods for screening for microcystin in environmental samples with greater sensitivity.

References

Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Feb 2, 2002·Applied and Environmental Microbiology·Melanie KaebernickBrett A Neilan
Feb 9, 2002·Journal of Clinical Laboratory Analysis·P MarkoulatosM Moncany
Apr 20, 2010·Applied and Environmental Microbiology·Hanna SipariKaarina Sivonen
Mar 18, 2011·Journal of Clinical Microbiology·Khushboo J NagdevHatim F Daginawala
May 15, 2012·Toxicology Letters·Yuan ZhouXiaodong Han
Jan 27, 2015·Environmental Monitoring and Assessment·Ellen P PreeceF Joan Hardy
Feb 22, 2016·Ecotoxicology and Environmental Safety·Maria do Carmo Bittencourt-OliveiraFlávio dos Santos
Aug 15, 2017·Toxicon : Official Journal of the International Society on Toxinology·Daljit VudathalaLisa Murphy

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