Detection of microsatellite instability in a panel of solid tumours with the Idylla MSI Test using extracted DNA

Journal of Clinical Pathology
Adrien PécriauxAnaïs Pujals

Abstract

During the last few years, determination of microstatellite instability (MSI) status has become a routine part of clinical practice, essentially to detect Lynch syndrome. Recently, MSI testing has increased with the development of immunotherapy and has expanded to a large panel of solid tumours. The aim of our work was to evaluate a fully automated system developed by Biocartis, the Idylla MSI Test, which performs an MSI analysis within 150 min. A comparison between pentaplex PCR, immunohistochemistry and Idylla MSI Test was performed in 53 colorectal carcinoma samples, 7 small intestine adenocarcinomas, 15 duodenal and pancreatic adenocarcinomas, 16 gastric tumours, 15 endometrial adenocarcinomas, 5 ovarian carcinomas and 4 cases of urinary tract tumours using extracted DNA. Limit-of-detection (LOD) experiment was also done using a commercial DNA known to harbour MSI phenotype. The overall sensitivity was 94% and the overall specificity was 100%. Two invalid and three false-negative results were observed. Our experiments showed that the amount of DNA loaded into the cartridge was decisive and should be superior to 25 ng. LOD comprised between 4% and 8%. Overall, we have demonstrated that the Idylla MSI Test is a rapid and vali...Continue Reading

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Dec 28, 2019·The Journal of Molecular Diagnostics : JMD·Karen ZwaenepoelPatrick Pauwels

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Citations

Mar 24, 2021·Virchows Archiv : an International Journal of Pathology·Iiris UkkolaAri Ristimäki
Jul 28, 2021·Journal of Clinical Pathology·Paola Rafaniello-RavieleMonica Marabelli

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