PMID: 11903796Mar 21, 2002Paper

Detection of mitochondrial DNA in living animal cells with fluorescence microscopy

Journal of Microscopy
M Dellinger, M Gèze

Abstract

The detection of mitochondrial DNA (mtDNA) in living human cells could be useful for understanding mitochondrial behaviour during cellular processes and pathological mtDNA depletions. However, until now, human mtDNA has not been visualized in living cells with fluorescence microscopy, although it has been easily detected in organisms with larger mtDNA. Previous reports have stated that mtDNA staining results in homogeneous fluorescence of mitochondria or that animal mitochondria are refractory to DAPI staining. This paper shows that mtDNA of cultured green monkey kidney CV-1 can be stained using a very low concentration of DAPI, then detected by a cooled Photometrics CCD camera with 14-bit resolution detection. Indeed, under these conditions CV-1 cells have small fluorescent spots in the cytoplasm that colocalize with mitochondria, even after mitochondrial movements, uncoupling by carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone and swelling. These observations have been reproduced for the human fibroblast foreskin cell line HS68. These results and known properties of DAPI as a specific DNA stain strongly suggest that mtDNA can be detected and visualized by fluorescence microscopy in human living cells, with potential devel...Continue Reading

Citations

Aug 28, 2003·Experimental Cell Research·Jessica MagnussonJan-Willem Taanman
Jul 11, 2006·Journal of Biomedical Optics·Silvia VersariSilvia Bradamante
May 26, 2004·BMC Biology·Francisco J IborraPeter R Cook
Feb 1, 2014·Reproductive Medicine and Biology·Katsumi KasashimaHitoshi Endo
May 30, 2006·Biochimica Et Biophysica Acta·Florence MalkaManuel Rojo
May 27, 2017·Journal of Biomedical Optics·Shilpa PantNanguang Chen
Dec 17, 2010·Chembiochem : a European Journal of Chemical Biology·Mei-Ling ZhengSatoshi Kawata
Oct 17, 2020·The Journal of Biological Chemistry·David L ProleNick S Jones
Jan 18, 2021·The Journal of Biological Chemistry·David L ProleNick S Jones
Sep 20, 2007·Analytical Chemistry·Marian NavratilEdgar A Arriaga
Feb 4, 2003·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Mario MateraVincenzo Savica
Jan 18, 2005·Experimental Cell Research·Neil AshleyJoanna Poulton

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