PMID: 9164485May 1, 1997Paper

Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations

Mutation Research
D SlamenováR Stĕtina

Abstract

Human cells (VH10 or Hep G2) and hamster cells V79 were exposed to different concentrations of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and the level of DNA lesions was evaluated by the DNA unwinding technique, alkaline elution of DNA and the comet assay. All three methods were able to detect the effects of MNNG but with a clear difference in sensitivity. At low concentrations of MNNG the most sensitive method appeared to be the comet assay. After the short-term treatment the comet assay was able to detect the lesions induced by MNNG at approx. 0.1 microgram/ml, alkaline elution of DNA at 1 microgram/ml and DNA unwinding at 1-2 micrograms/ml. MNNG treated VH10 cells, human lymphocytes and V79 cells were also tested cytogenetically, confirming that MNNG induced chromosomal aberrations at concentrations > 1 microgram/ml in VH10 cells (short-term treatment): > 0.2 microgram/ml in V79 cells (long-term treatment) and > 8 micrograms/ml in human lymphocytes (long-term treatment). In some experiments we tried to increase the level of MNNG-induced DNA breaks with help of DNA repair inhibitors cytosine arabinoside (Ara C) and hydroxyurea (HU) which were applied either after or during MNNG treatment. Our results showed that the level o...Continue Reading

Citations

Dec 24, 2003·Teratogenesis, Carcinogenesis, and Mutagenesis·Elizabeth D WagnerMichael J Plewa
Oct 20, 1998·Somatic Cell and Molecular Genetics·T T PuckG Yohrling
Sep 28, 1998·Mutation Research·A Cebulska-WasilewskaD Anderson
Oct 22, 2003·Aquatic Toxicology·Zeljko Jaksić, Renato Batel
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde
Mar 1, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I Kamer, B Rinkevich
May 22, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T O'BrienK Mitchell
Jan 6, 1999·Mutation Research·E HorváthováA R Collins
May 9, 2001·Mutation Research·M PavlicaD Papes
Apr 29, 2009·International Journal of Molecular Sciences·Lim Chin Keong, Ahmad Sukari Halim
Dec 4, 2003·Mutation Research·Antonina Cebulska-Wasilewska
Oct 23, 2010·Journal of Photochemistry and Photobiology. B, Biology·Soňa JantováVlasta Brezová
Dec 17, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Joachim B HavlaNorbert H Kleinsasser
Nov 2, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Roberta MeschiniFabrizio Palitti
Aug 25, 2004·Mutation Research·Alena GábelováPeter B Farmer
Aug 15, 2006·Journal of Photochemistry and Photobiology. B, Biology·Sona JantováJuraj Lábaj
Dec 31, 2005·Toxicology Letters·Monika LazarováDarina Slamenová
Feb 18, 2014·International Journal of Radiation Biology·Sudhir ChandnaSiamak Haghdoost
Mar 17, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E HorváthováL Wsólová
Aug 22, 2016·Analytical and Bioanalytical Chemistry·Liyuan MaMing Su
Jan 22, 2005·Mutation Research·Katarína HorváthováAnna Vachálková

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