Detection of molecular markers of antiviral resistance in influenza A (H5N1) viruses using a pyrosequencing method.

Antimicrobial Agents and Chemotherapy
Varough M DeydeLarisa V Gubareva

Abstract

Resistance of influenza viruses to antiviral drugs can emerge following medication or may result from natural variation. Two classes of anti-influenza virus drugs targeting either the M2 protein (amantadine and rimantadine) or neuraminidase (NA; oseltamivir and zanamivir) are currently licensed. These drugs are expected to be important in controlling the early stages of a potential pandemic. In the present study, we describe how a pyrosequencing method can be used to rapidly detect established molecular markers of resistance to M2 blockers and NA inhibitors in influenza A (H5N1) viruses. The residues L26, V27, A30, S31, and G34 in the M2 protein were targeted for pyrosequencing. The NA residues for pyrosequencing analysis included the established markers of drug resistance (H274 and N294), as well as residues of less certain relevance (V116, I117, Q136, K150, and I222). A single pair of pyro-reverse transcription (RT)-PCR primers was designed to allow amplification of an approximately 600-nucleotide-long amplicon of the NA genes of H5N1 viruses from various clades/subclades associated with infections in humans. The sensitivity of the assay was demonstrated by the successful pyrosequencing of RNA extracted from samples of serial...Continue Reading

References

Oct 1, 1986·The Journal of Antimicrobial Chemotherapy·A J HayM H Smith
Oct 23, 1997·Nature·J C de JongW L Lim
Jul 31, 1998·Vaccine·E C ClaasA D Osterhaus
Mar 11, 2000·Lancet·L V GubarevaF G Hayden
Oct 31, 2001·Antiviral Research·Larisa V GubarevaFrederick G Hayden
Jan 25, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Guy BoivinHarold Bernatchez
Sep 10, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Arnold S MontoSuzanne E Ohmit
Feb 15, 2005·The Journal of Antimicrobial Chemotherapy·Penelope WardRegina Dutkowski
Sep 30, 2005·The New England Journal of Medicine·Anne Moscona
Oct 18, 2005·Nature·Q Mai LeYoshihiro Kawaoka
Dec 24, 2005·The New England Journal of Medicine·Menno D de JongJeremy Farrar
Feb 4, 2006·JAMA : the Journal of the American Medical Association·Rick A BrightAlexander I Klimov
May 17, 2006·The Journal of Infectious Diseases·Chung-Lam CheungHonglin Chen
Jul 4, 2006·Antiviral Research·Frederick G Hayden
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·M A Rameix-WeltiN Naffakh
Nov 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mariana BazGuy Boivin
Jan 19, 2007·The New England Journal of Medicine·Reiko SaitoHiroshi Suzuki
Jun 16, 2007·The Journal of Infectious Diseases·Varough M DeydeAlexander I Klimov
Dec 7, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Olivier Ferraris, Bruno Lina
Jan 18, 2008·The New England Journal of Medicine·UNKNOWN Writing Committee of the Second World Health Organization Consultation on Clinical Aspects of Human Infection with Avian InfTimothy M Uyeki
Feb 7, 2008·Emerging Infectious Diseases·Jennifer L McKimm-BreschkinMichael A Johnson
May 1, 2008·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·A LackenbyM C Zambon
May 16, 2008·Nature·Patrick J CollinsSteven J Gamblin
Jun 19, 2008·Journal of Medicinal Chemistry·Lily S ChengJ Andrew McCammon
Aug 22, 2008·Journal of Virology·Xiaojin XuIan A Wilson

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Citations

Oct 19, 2010·Avian Pathology : Journal of the W.V.P.A·Matteo Samuele PizzutoGiovanni Cattoli
Dec 24, 2009·Antimicrobial Agents and Chemotherapy·Varough M DeydeLarisa V Gubareva
Apr 12, 2012·Clinical Microbiology Reviews·Swati Kumar, Kelly J Henrickson
Feb 15, 2012·Journal of Visualized Experiments : JoVE·Pawan KumarSubramaniam Malarkannan
Dec 22, 2011·Critical Reviews in Clinical Laboratory Sciences·James B MahonyMarek Smieja
May 12, 2010·Expert Review of Anti-infective Therapy·Ruixue Wang, Jeffery K Taubenberger
Jul 8, 2009·Expert Review of Molecular Diagnostics·Varough M Deyde, Larisa V Gubareva
Aug 3, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·C RenaudL Corey
Jul 10, 2010·Antiviral Research·Repon Kumer SahaTakashi Suzuki
Mar 23, 2010·Archives of Medical Research·Carlos F AriasSusana López
Nov 7, 2009·Clinics in Laboratory Medicine·Wenjuan Wu, Yi-Wei Tang
Jan 4, 2013·Influenza and Other Respiratory Viruses·Michael G Ison
Jun 14, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·W RutvisuttinuntS Fernandez
Apr 20, 2013·The Journal of Molecular Diagnostics : JMD·Erhard van der VriesMartin Schutten
Feb 4, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Fabio BenziRoberto Biassoni
Jul 8, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Miguel E Quiñones-MateuMiguel A Martinez
Dec 14, 2012·Antiviral Chemistry & Chemotherapy·Andreas J KeselRaymond F Schinazi
Jul 20, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hana A PawestriMenno D de Jong

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