Detection of moniliformin in maize using capillary zone electrophoresis

Food Additives and Contaminants
C M Maragos

Abstract

Moniliformin is a mycotoxin produced by certain fungi pathogenic to maize. It is capable of causing disease in domestic animals, possibly through inhibition of pyruvate dehydrogenase. Testing for MON commonly involves extraction of maize, isolation of moniliformin using solid-phase extraction columns and detection with high-performance liquid chromatography (HPLC) or gas chromatography. A capillary zone electrophoresis-diode array detection (CZE-DAD) method for determination of moniliformin in maize is reported. The extraction and isolation procedures are similar to those of a commonly used HPLC method, while the detection step requires only 10 min. Sixty-three samples of maize were tested by an established HPLC method using absorbance at 229 nm (HPLC-ultraviolet light) and by the CZE-DAD method. The limit of detection of the CZE-DAD method was 0.1 microg MON g(-1) maize compared with 0.05 microg g(-1) for the HPLC-ultraviolet light method. The CZE-DAD method gave good agreement with the HPLC-ultraviolet light method for samples tested at levels up to 1500 microg g(-1), with a linear regression of r(2) = 0.996.

References

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Citations

Mar 21, 2012·Journal of Agricultural and Food Chemistry·Katharina Walburga von BargenHans-Ulrich Humpf
Dec 14, 2006·Food Additives and Contaminants·M AppellC M Maragos
Feb 16, 2008·Critical Reviews in Food Science and Nutrition·Marika Jestoi
Oct 18, 2007·Electrophoresis·Virginia García-Cañas, Alejandro Cifuentes
Mar 2, 2018·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Lutz Edler
Sep 14, 2006·Journal of Agricultural and Food Chemistry·Anne E DesjardinsRobert H Proctor
Jun 23, 2005·Journal of Agricultural and Food Chemistry·Soo-Hyun ChungLloyd B Bullerman

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

[UNK] chromatography system

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