Detection of morbillivirus infection by RT-PCR RFLP analysis in cetaceans and carnivores

Journal of Virological Methods
Federica VernaMaria Goria

Abstract

Morbillivirus genus comprises several members related to specific hosts, such as canine distemper virus (CDV) and cetacean morbillivirus (CeMV) in which the dolphin morbillivirus (DMV) is included. Both CDV and DMV are able to cause serious outbreak associated with high morbidity and mortality representing an important conservation threat for terrestrial and aquatic mammalian species. This paper describes a new RT-PCR RFLP technique based on a RT-PCR with degenerate primers targeting a 287 bp fragment located on the conserved N terminus of the morbillivirus NP gene, followed by MseI RFLP, in order both to confirm the detection of the virus and to distinguish DMV from CDV. Both carnivores and cetaceans tissues (brain, lung and lymph node) presenting evidence of morbillivirus infection (MI) were analyzed. RT-PCR positive samples were typed by RFLP analysis and then sequenced to confirm the RFLP results. This method was applied during the last morbillivirus cetacean die-off occurred in the Mediterranean basin in 2013, when there was the urgent need of a rapid and economic method to investigate among causes of death on stranded cetaceans. This new technique has proved to be a valuable, reliable, simple and relatively inexpensive di...Continue Reading

References

Dec 24, 2014·Viruses·Marie-Françoise Van BressemJames F X Wellehan

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Citations

Jan 26, 2018·Journal of Wildlife Diseases·Maria Domenica PintoreBarbara Iulini
Oct 3, 2020·PloS One·Giuliano GarofoloCarla Grattarola
Jan 23, 2019·Emerging Infectious Diseases·Iolanda PadalinoAntonio Petrella
Jun 13, 2019·Diseases of Aquatic Organisms·Alessandra PautassoCristina Casalone
Dec 2, 2020·Scientific Reports·Francesco CeruttiSimone Peletto
Apr 26, 2021·Journal of Comparative Pathology·Sandro MazzariolCristina Casalone
May 1, 2021·Animals : an Open Access Journal From MDPI·Federica GiordaCristina Casalone

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