Detection of Müllerian inhibiting substance in biological samples by a solid phase sandwich radioimmunoassay

Endocrinology
E C NecklawsP K Donahoe

Abstract

Müllerian inhibiting substance (MIS) is a 140,000-dalton glycoprotein responsible for regression of Müllerian ducts in a male embryo. It has recently been demonstrated that MIS inhibits the growth of tumors in vivo and in vitro. In this study, we have constructed a sensitive, solid phase sandwich RIA using monoclonal antibodies raised to bovine MIS. The amount of MIS detected was based on the protein concentration of Green 3, the most purified fraction of MIS available. The assay could detect 20 ng Green 3 or 0.14 pmol in physiological samples. There was no evidence of cross-reactivity of the antibodies raised to bovine MIS with chicken, rat, mouse, or human MIS.

Citations

Mar 1, 1993·Molecular Reproduction and Development·K M KelleyJ Ilan
Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J EpsteinP K Donahoe
Oct 1, 1986·Molecular and Cellular Endocrinology·M TakahashiP K Donahoe
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Jun 1, 1993·Microscopy Research and Technique·E A CatlinP K Donahoe
Nov 1, 1991·Trends in Endocrinology and Metabolism : TEM·N JossoJ Y Picard
Jun 11, 2019·Analytical and Bioanalytical Chemistry·Yu WangXiaowei He
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