Detection of multiple human herpes viruses by DNA microarray technology

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
Z Földes-PappPeter Wutzler

Abstract

The detailed characterization of virus DNA is a challenge, and the genotyping that has been achieved to date has only been possible because researchers have sent a great deal of time and effort to do so. Instead of the simultaneous detection of hundreds of viruses on a single high-density DNA-chip at very high costs per chip, we present here an alternative approach using a well-designed and tailored microarray which can establish whether or not a handful of viral genes are present in a clinical sample. In this study we applied a new concept of microarray-based, optimized and robust biochemistry for molecular diagnostics of the herpesviruses. For comparison, all samples were genotyped using standard procedures. The biochemical procedure of a knowledge-based, low-density microarray was established based on the molecular diagnostics of human herpes viruses: herpes simplex virus (HSV) HSV-1, HSV-2, varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and HHV-6. The study attempted to optimize parameters of microarray design, surface chemistry, oligonucleotide probe spotting, sample labeling and DNA hybridization to the developed DNA microarray. The results of 12 900 hybridization reactions on about 150 con...Continue Reading

References

Oct 1, 1990·Journal of Clinical Microbiology·A TelentiT F Smith
Sep 1, 1990·Journal of Medical Virology·R F JarrettD E Onions
Jan 1, 1990·Medical Microbiology and Immunology·H KimuraT Morishima
Mar 1, 1994·Journal of Virology·S KarlinG A Schachtel
May 1, 1997·Journal of Virology·Y InoueS Fujita
Jul 26, 2000·Transplantation·I LautenschlagerK Höckerstedt
Nov 9, 2000·The New England Journal of Medicine·M LuppiG Torelli
Nov 10, 2000·Nucleic Acids Research·R PenchovskyJ S McCaskill
Aug 21, 2001·Molecular Cell·A CarfíD C Wiley
Nov 29, 2001·Journal of Clinical Microbiology·P MarkoulatosJ Kourea-Kremastinou
Nov 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·David WangJoseph L DeRisi
Jun 5, 2003·Pharmacology & Therapeutics·Santo LandolfoDavid Lembo
Oct 3, 2003·The New England Journal of Medicine·Richard Ambinder
Nov 5, 2003·Advanced Drug Delivery Reviews·Judy A Garner
Dec 20, 2003·Current Pharmaceutical Biotechnology·Hans-Martin StriebelZeno Földes-Papp

❮ Previous
Next ❯

Citations

Jan 6, 2009·BMC Microbiology·Maria Palka-SantiniOleg Krut
Nov 30, 2010·The Veterinary Journal·Nicholas Bexfield, Paul Kellam
Mar 28, 2007·Heart Failure Clinics·Neil E BowlesJeffrey A Towbin
Aug 2, 2006·Transfusion Medicine·J Petrik
Feb 22, 2005·Biochemical and Biophysical Research Communications·Junko H OhyashikiKazuma Ohyashiki
Sep 8, 2004·Experimental and Molecular Pathology·Hans-Martin StriebelAxel Stelzner
Nov 22, 2005·Experimental and Molecular Pathology·Masahiro FujimuroHideyoshi Yokosawa
Nov 30, 2005·BioTechniques·Concepcion Conejero-GoldbergE Fuller Torrey
Aug 23, 2005·Memórias do Instituto Oswaldo Cruz·Meltem Yalinay CirakSukru Sindel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Pharmaceutical Biotechnology
Hans-Martin StriebelZeno Földes-Papp
Proceedings of the National Academy of Sciences of the United States of America
David WangJoseph L DeRisi
© 2022 Meta ULC. All rights reserved