Detection of multiple organisms based on the distance-dependent optical properties of gold nanoparticle and dark-field microscopy

Talanta
Nongthombam BobyPallab Chaudhuri

Abstract

Owing to their unique physical and chemical properties like stability, non-toxic, biocompatibility and feasible to modification with various biomolecules, gold nanoparticle has become a versatile nanomaterial in the field of therapeutic, diagnostic and analytical studies. Various surface plasmon resonance based pathogen detection systems, relying on change in colour, have been proposed. However, all the approaches developed so far were designed for the detection of a single pathogen. In the present study, we have designed a new colorimetric approach based on distant-dependent properties of gold nanoparticle for the detection of multiple targets. A modified multiplex asymmetric PCR in which a universal primer amplifies the multiple targets with the same efficiency was performed. The Limit of detection (LOD) of the designed visual assay is 10 pg of Brucella and Leptospira target DNA and 100 pg of Bovine herpes virus-1 (BoHV-1) target DNA. LOD of 0.5 pg, 0.7 pg and 3.8 pg for Brucella, Leptospira and BoHV-1 respectively was obtained spectrophotometrically. A study on dark field microscopy as a qualitative supporting detection system has also been presented in this study. The designed assay has advantages over earlier reports in te...Continue Reading

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Citations

Jan 12, 2021·Animal Biotechnology·Syed Atif AliPallab Chaudhuri
Feb 24, 2021·Analytical Chemistry·Peng Fei GaoCheng Zhi Huang
Jun 24, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bicong ZhouChangmin Yu

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